Effect of inositol hexakisphosphate on the spectroscopic properties of the nitric oxide derivative of ferrous horse and bovine hemoglobin

Paolo Ascenzi, Massimo Coletta, Alessandro Desideri, Francesca Polizio, Saverio Giovanni Condo', Bruno Giardina

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of inositol hexakisphosphate (IHP) on the spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous horse and bovine hemoglobin (Hb) has been investigated. In the absence of IHP, the nitric oxide derivative of ferrous horse Hb shows spectroscopic properties similar to those of the corresponding derivative of ferrous human Hb that are generally taken as typical of the high affinity state of tetrametric hemoproteins. Similar to human Hb, the addition of IHP to the nitric oxide derivative of ferrous horse Hb induces a transition toward a species characterized by spectral properties typical of the low affinity state of hemoglobins. Nevertheless, the equilibrium constant for IHP binding to the nitric oxide derivative of ferrous horse Hb (= 1.5 × 102 M-1) is much lower than that reported for the association of the polyphosphate to the same derivative of ferrous human Hb (d3 × 105 M-1). Conversely, the spectroscopic properties of the nitric oxide derivative of ferrous bovine Hb are characteristic of the low affinity state of tetrameric hemoproteins, both in the absence and in the presence of IHP. These results, taken together with the behavior of the nitric oxide derivative of ferrous human Hb, provide further evidence for the peculiar oxygen binding properties of horse and bovine Hb.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalJournal of Inorganic Biochemistry
Volume40
Issue number2
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Effect of inositol hexakisphosphate on the spectroscopic properties of the nitric oxide derivative of ferrous horse and bovine hemoglobin'. Together they form a unique fingerprint.

Cite this