Effect of interferon-gamma on hla class II antigen expression and sensitivity to prostaglandin E1 by normal and leukemic myeloid progenitors

Massimo Aglietta, Wanda Piacibello, Alessandra Stacchini, Fiorella Sanavio, Vittorio Infelise, Luigi Resegotti, Felice Gavosto

Research output: Contribution to journalArticlepeer-review

Abstract

Chronic myelogenous leukemia (CML) granulomonocyte committed progenitors (CFUGM) are markedly less sensitive than normal progenitors to the inhibitory action of prostaglandin E (PGE). This phenomenon has been ascribed to their abnormal expression of HLA class II (mainly DR) determinants. Since interferon gamma (IFN-γ) is a potent inducer of the expression of HLA class II (DR and to a lesser extent DQ) antigens, we have sought to determine the extent to which this agent can modulate both the antigenic pattern of normal and leukemic progenitors and their sensitivity to PGE 1. 72-h preincubation of normal and CML bone marrow cells with or without IFN-γ does not significantly change DR and DQ expression by CFU-GM. Pre-incubation for 72 h with and without IFN-γ produces the following changes in PGE 1 sensitivity: (1) normal CFU-GM lose some sensitivity to PGE 1. This is only marginally counteracted by the presence of IFN-γ. (2) CML CFU-GM, preincubated with IFN-γ regain a significant sensitivity to high concentrations of PGE 1. Our data confirm the expression of DR molecules on normal and leukemic progenitors. They also show that, although incubation with IFN-γ for 72 h in a liquid culture system does not significantly affect the expression of HLA class II molecules by progenitor cells, it may increase their sensitivity to PGE, particularly in the case of CML CFU-GM. Thus expression of HLA class II antigens and sensitivity to PGE may be dissociated.

Original languageEnglish
Pages (from-to)299-303
Number of pages5
JournalLeukemia Research
Volume12
Issue number4
DOIs
Publication statusPublished - 1988

Keywords

  • histocompatibility antigens
  • in vitro
  • Interferon-gamma
  • leukemia
  • myelopoiesis
  • prostaglandins

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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