Effect of interleukin-10 on the production of tumor necrosis factor-alpha by peripheral blood mononuclear cells from patients with chronic heart failure

Aidan P. Bolger, Rakesh Sharma, Stephan Von Haehling, Wolfram Doehner, Brian Oliver, Mathias Rauchhaus, Andrew J S Coats, Ian M. Adcock, Stefan D. Anker

Research output: Contribution to journalArticle

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Abstract

Chronic heart failure (HF) is a state of inflammatory immune activation characterized by elevated circulating levels of tumor necrosis factor-α (TNF-α). Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine that inhibits TNF-α production and lessens endotoxin bioactivity. It is not known whether IL-10 reduces lipopolysaccharide (LPS) stimulated TNF-α production of peripheral blood mononuclear cells (PBMCs) from patients with chronic HF. PBMCs were isolated from 15 patients with chronic HF (New York Heart Association functional class 3.0 ± 0.2, left ventricular ejection fraction 30 ± 2%, peak oxygen consumption 18.1 ± 0.8 ml/kg/min) and 15 healthy control subjects and stimulated with 1 and 10 ng/ml LPS for 24 hours with or without prior addition of IL-10 (10 ng/ml). TNF-α was quantified in cell-free supernatants by an enzyme-linked immunosorbent assay. TNF-α, soluble TNF receptors, IL-10, and LPS were quantified in plasma. LPS stimulated TNF-α production was highest in those patients in New York Heart Association class II (p

Original languageEnglish
Pages (from-to)384-389
Number of pages6
JournalThe American Journal of Cardiology
Volume90
Issue number4
DOIs
Publication statusPublished - Aug 15 2002

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Interleukin-10
Blood Cells
Heart Failure
Tumor Necrosis Factor-alpha
Lipopolysaccharides
Tumor Necrosis Factor Receptors
Endotoxins
Oxygen Consumption
Stroke Volume
Healthy Volunteers
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay
Cytokines

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Effect of interleukin-10 on the production of tumor necrosis factor-alpha by peripheral blood mononuclear cells from patients with chronic heart failure. / Bolger, Aidan P.; Sharma, Rakesh; Von Haehling, Stephan; Doehner, Wolfram; Oliver, Brian; Rauchhaus, Mathias; Coats, Andrew J S; Adcock, Ian M.; Anker, Stefan D.

In: The American Journal of Cardiology, Vol. 90, No. 4, 15.08.2002, p. 384-389.

Research output: Contribution to journalArticle

Bolger, Aidan P. ; Sharma, Rakesh ; Von Haehling, Stephan ; Doehner, Wolfram ; Oliver, Brian ; Rauchhaus, Mathias ; Coats, Andrew J S ; Adcock, Ian M. ; Anker, Stefan D. / Effect of interleukin-10 on the production of tumor necrosis factor-alpha by peripheral blood mononuclear cells from patients with chronic heart failure. In: The American Journal of Cardiology. 2002 ; Vol. 90, No. 4. pp. 384-389.
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