Abstract
Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. Results and Conclusion: In this cellular model LT decreased mRNA defensins’ expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time.
Original language | English |
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Pages (from-to) | 326-329 |
Number of pages | 4 |
Journal | Protein and Peptide Letters |
Volume | 25 |
Issue number | 4 |
DOIs | |
Publication status | Published - Jan 1 2018 |
Keywords
- Gene expression
- Innate immunity
- Laser therapy
- Oral mucositis
- Real time PCR
- β-defensins
ASJC Scopus subject areas
- Structural Biology
- Biochemistry