Effect of laser therapy on defensins’ gene expression in TR146 Epithelial cell line

Luisa Zupin, Katia Rupel, Giulia Ottaviani, Augusto Poropat, Roberto Di Lenarda, Matteo Biasotto, Sergio Crovella

Research output: Contribution to journalArticlepeer-review


Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. Results and Conclusion: In this cellular model LT decreased mRNA defensins’ expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalProtein and Peptide Letters
Issue number4
Publication statusPublished - Jan 1 2018


  • Gene expression
  • Innate immunity
  • Laser therapy
  • Oral mucositis
  • Real time PCR
  • β-defensins

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry


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