Effect of laser therapy on defensins’ gene expression in TR146 Epithelial cell line

Luisa Zupin; Katia Rupel; Giulia Ottaviani; Augusto Poropat; Roberto Di Lenarda; Matteo Biasotto; Sergio Crovella

doi:10.2174/0929866525666180221123249

Effect of laser therapy on defensins’ gene expression in TR146 Epithelial cell line

Luisa Zupin, Katia Rupel, Giulia Ottaviani, Augusto Poropat, Roberto Di Lenarda, Matteo Biasotto, Sergio Crovella

IRCCS pediatrico Burlo Garofolo

Research output: Contribution to journal › Article › peer-review

Abstract

Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. Results and Conclusion: In this cellular model LT decreased mRNA defensins’ expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time.

Original language	English
Pages (from-to)	326-329
Number of pages	4
Journal	Protein and Peptide Letters
Volume	25
Issue number	4
DOIs	https://doi.org/10.2174/0929866525666180221123249
Publication status	Published - Jan 1 2018

Keywords

Gene expression
Innate immunity
Laser therapy
Oral mucositis
Real time PCR
β-defensins

ASJC Scopus subject areas

Structural Biology
Biochemistry

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title = "Effect of laser therapy on defensins{\textquoteright} gene expression in TR146 Epithelial cell line",

abstract = "Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. Results and Conclusion: In this cellular model LT decreased mRNA defensins{\textquoteright} expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time.",

keywords = "Gene expression, Innate immunity, Laser therapy, Oral mucositis, Real time PCR, β-defensins",

author = "Luisa Zupin and Katia Rupel and Giulia Ottaviani and Augusto Poropat and {Di Lenarda}, Roberto and Matteo Biasotto and Sergio Crovella",

year = "2018",

month = jan,

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doi = "10.2174/0929866525666180221123249",

language = "English",

volume = "25",

pages = "326--329",

journal = "Protein and Peptide Letters",

issn = "0929-8665",

publisher = "Bentham Science Publishers B.V.",

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TY - JOUR

T1 - Effect of laser therapy on defensins’ gene expression in TR146 Epithelial cell line

AU - Zupin, Luisa

AU - Rupel, Katia

AU - Ottaviani, Giulia

AU - Poropat, Augusto

AU - Di Lenarda, Roberto

AU - Biasotto, Matteo

AU - Crovella, Sergio

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. Results and Conclusion: In this cellular model LT decreased mRNA defensins’ expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time.

AB - Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. Results and Conclusion: In this cellular model LT decreased mRNA defensins’ expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β-defensins production immediately after treatment, while its efficacy decreases over time.

KW - Gene expression

KW - Innate immunity

KW - Laser therapy

KW - Oral mucositis

KW - Real time PCR

KW - β-defensins

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