Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis

Giovanni Camussi, Giro Tetta, Federico Bussolino, Ernesto Turello, Jan Brentjens, Giuseppe Montrucchio, Giuseppe Andres

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Phytohemagglutinin (PHA), a leukocyte mitogen, induces a lymphocyte and blast cell glomerulonephritis in rat renal allografts (Cell Immunol 13:146, 1974). The aim of this study was to assess whether PHA similarly enhances rabbit monocyte-dependent experimental, acute immune complex glomerulonephritis, and whether this effect is associated with local release of interleukin-1 (IL-1) and tumor necrosis factor (TNF). Rabbits with experimental acute serum sickness (AcSS: Group I) had focal proliferative and exudative glomerulonephntis with immune deposits, scattered subepithelial electron-dense deposits (humps) mild and transient proteinuria, normal creatinine clearance and slightly increased production of IL-1 and TNF from isolated glomeruli Rabbits with AcSS and injected with PHA (Group II) developed severe lymphocyte and blast cell glomerulonephritis with diffuse endothelial damage; immune deposits were significantly reduced, focal subepithelial electron-dense deposits were absent, proteinuria was increased, creatinine clearance was decreased and production of IL-1 and TNF was markedly augmented as compared to rabbits in Group I. Rabbits with AcSS and injected with IL-1β and TNFα (Group V) had lesions comparable to those seen in Group II. These results show that PHA, IL-1 and TNF enhance the severity of acute immune complex glomerulonephritis, presumably by activating glomerular endothelial and mesangial cells and resident or infiltrated leukocytes.

Original languageEnglish
Pages (from-to)1047-1055
Number of pages9
JournalKidney International
Volume38
Issue number6
Publication statusPublished - Dec 1990

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Glomerulonephritis
Antigen-Antibody Complex
Interleukin-1
Phytohemagglutinins
Leukocytes
Tumor Necrosis Factor-alpha
Rabbits
Proteinuria
Creatinine
Serum Sickness
Lymphocytes
Electrons
Mesangial Cells
Mitogens
Allografts
Monocytes
Endothelial Cells
Kidney

ASJC Scopus subject areas

  • Nephrology

Cite this

Camussi, G., Tetta, G., Bussolino, F., Turello, E., Brentjens, J., Montrucchio, G., & Andres, G. (1990). Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis. Kidney International, 38(6), 1047-1055.

Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis. / Camussi, Giovanni; Tetta, Giro; Bussolino, Federico; Turello, Ernesto; Brentjens, Jan; Montrucchio, Giuseppe; Andres, Giuseppe.

In: Kidney International, Vol. 38, No. 6, 12.1990, p. 1047-1055.

Research output: Contribution to journalArticle

Camussi, G, Tetta, G, Bussolino, F, Turello, E, Brentjens, J, Montrucchio, G & Andres, G 1990, 'Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis', Kidney International, vol. 38, no. 6, pp. 1047-1055.
Camussi G, Tetta G, Bussolino F, Turello E, Brentjens J, Montrucchio G et al. Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis. Kidney International. 1990 Dec;38(6):1047-1055.
Camussi, Giovanni ; Tetta, Giro ; Bussolino, Federico ; Turello, Ernesto ; Brentjens, Jan ; Montrucchio, Giuseppe ; Andres, Giuseppe. / Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis. In: Kidney International. 1990 ; Vol. 38, No. 6. pp. 1047-1055.
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