Effect of leukocyte stimulation on rabbit immune complex glomerulonephritis

Giovanni Camussi, Giro Tetta, Federico Bussolino, Ernesto Turello, Jan Brentjens, Giuseppe Montrucchio, Giuseppe Andres

Research output: Contribution to journalArticlepeer-review

Abstract

Phytohemagglutinin (PHA), a leukocyte mitogen, induces a lymphocyte and blast cell glomerulonephritis in rat renal allografts (Cell Immunol 13:146, 1974). The aim of this study was to assess whether PHA similarly enhances rabbit monocyte-dependent experimental, acute immune complex glomerulonephritis, and whether this effect is associated with local release of interleukin-1 (IL-1) and tumor necrosis factor (TNF). Rabbits with experimental acute serum sickness (AcSS: Group I) had focal proliferative and exudative glomerulonephntis with immune deposits, scattered subepithelial electron-dense deposits (humps) mild and transient proteinuria, normal creatinine clearance and slightly increased production of IL-1 and TNF from isolated glomeruli Rabbits with AcSS and injected with PHA (Group II) developed severe lymphocyte and blast cell glomerulonephritis with diffuse endothelial damage; immune deposits were significantly reduced, focal subepithelial electron-dense deposits were absent, proteinuria was increased, creatinine clearance was decreased and production of IL-1 and TNF was markedly augmented as compared to rabbits in Group I. Rabbits with AcSS and injected with IL-1β and TNFα (Group V) had lesions comparable to those seen in Group II. These results show that PHA, IL-1 and TNF enhance the severity of acute immune complex glomerulonephritis, presumably by activating glomerular endothelial and mesangial cells and resident or infiltrated leukocytes.

Original languageEnglish
Pages (from-to)1047-1055
Number of pages9
JournalKidney International
Volume38
Issue number6
Publication statusPublished - Dec 1990

ASJC Scopus subject areas

  • Nephrology

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