Effect of modified alkaline supplementation on syngenic melanoma growth in CB57/BL mice

Tommaso Azzarito, Luana Lugini, Enrico Pierluigi Spugnini, Rossella Canese, Alessio Gugliotta, Stefano Fidanza, Stefano Fais

Research output: Contribution to journalArticle

Abstract

Tumor extracellular acidity is a hallmark of malignant cancers. Thus, in this study we evaluated the effects of the oral administration of a commercially available water alkalizer (Basenpulver1) (BP) on tumor growth in a syngenic melanoma mouse model. The alkalizer was administered daily by oral gavage starting one week after tumor implantation in CB57/BL mice. Tumors were calipered and their acidity measured by in vivo MRI guided 31P MRS. Furthermore, urine pH was monitored for potential metabolic alkalosis. BP administration significantly reduced melanoma growth in mice; the optimal dose in terms of tolerability and efficacy was 8 g/l (p<0.05). The in vivo results were supported by in vitro experiments, wherein BP-treated human and murine melanoma cell cultures exhibited a dose-dependent inhibition of tumor cell growth. This investigation provides the first proof of concept that systemic buffering can improve tumor control by itself and that this approach may represent a new strategy in prevention and/or treatment of cancers.

Original languageEnglish
Article numbere0159763
JournalPLoS One
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Azzarito, T., Lugini, L., Spugnini, E. P., Canese, R., Gugliotta, A., Fidanza, S., & Fais, S. (2016). Effect of modified alkaline supplementation on syngenic melanoma growth in CB57/BL mice. PLoS One, 11(7), [e0159763]. https://doi.org/10.1371/journal.pone.0159763