Effect of mother's age and pathology on functional behavior of amniotic mesenchymal stromal cells-Hints for bone regeneration

Maria Matteo, Elisa Beccia, Annalucia Carbone, Stefano Castellani, Lucio Milillo, Dorina Lauritano, Sante Di Gioia, Antonella Angiolillo, Massimo Conese

Research output: Contribution to journalArticlepeer-review


Human amnion-derived mesenchymal stromal cells (hAMSCs) are used increasingly in regenerative medicine applications, including dentistry. The aim of this study was to evaluate if hAMSCs from aged and pathological mothers could be affected in their phenotype and functional behavior. hAMSCs were isolated from placentas of women aged younger than 40 years (Group 1, n = 7), older than 40 years (Group 2, n = 6), and with pre-eclampsia (Group 3, n = 5). Cell yield and viability were assessed at isolation (p0). Cell proliferation was evaluated from p0 to p5. Passage 2 was used to determine the phenotype, the differentiation capacity, and the adhesion to machined and sandblasted titanium disks. hAMSCs recovered from Group 3 were fewer than in Group 1. Viability and doubling time were not different among the three groups. Percentages of CD29+ cells were significantly lower in Group 3, while percentages of CD73+ cells were significantly lower in Groups 2 and 3 as compared with Group 1. hAMSCs from Group 2 showed a significant lower differentiation capacity towards chondrogenic and osteogenic lineages. hAMSCs from Group 3 adhered less to titanium surfaces. In conclusion, pathology can affect hAMSCs in phenotype and functional behavior and may alter bone regeneration capacities.

Original languageEnglish
Article number3471
JournalApplied Sciences (Switzerland)
Issue number17
Publication statusPublished - Sep 1 2019


  • Adhesion
  • Amnion
  • Differentiation
  • Mesenchymal stem cells
  • Pre-eclampsia
  • Titanium

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


Dive into the research topics of 'Effect of mother's age and pathology on functional behavior of amniotic mesenchymal stromal cells-Hints for bone regeneration'. Together they form a unique fingerprint.

Cite this