Effect of myocardial ischaemia-reperfusion on granulocyte elastase release

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

During extracorporeal circulation, activation and degranulation of neutrophil granulocytes occur, with subsequent massive release of elastase, a neutral protease stored in the azurophil granules. Neutrophil granulocytes and oxygen-derived free radicals are intimately involved in the occurrence of reperfusion injury, which affects especially the lungs and the myocardium. In this study we evaluated changes in the leucocyte count and of the plasma elastase levels in the coronary circulation during open heart surgery. We collected blood samples from the radial artery and the coronary sinus before skin incision, after onset of cardiopulmonary bypass and 5, 15, 30, 60, 120 minutes after the release of the aortic cross-clamp. The leucocyte count did not change significantly during the operation and no difference in leucocyte count was found between the radial artery and the coronary sinus. Plasma elastase levels significantly increased after the onset of cardiopulmonary bypass (P = 0.007) and after the release of the aortic cross-clamp (P = 0.001). Moreover, significantly higher values were found in the coronary sinus than in the radial artery 5 (P = 0.04), 15 (P = 0.02) and 30 (P = 0.02) minutes after the release of the aortic cross-clamp. We assume, therefore, that during the early phase of reperfusion, degranulation of neutrophil granulocytes occurs in the coronary circulation.

Original languageEnglish
Pages (from-to)187-190
Number of pages4
JournalAnaesthesia and Intensive Care
Volume20
Issue number2
Publication statusPublished - 1992

Fingerprint

Myocardial Reperfusion
Leukocyte Elastase
Radial Artery
Coronary Sinus
Pancreatic Elastase
Leukocyte Count
Granulocytes
Myocardial Ischemia
Coronary Circulation
Cardiopulmonary Bypass
Neutrophils
Neutrophil Activation
Extracorporeal Circulation
Reperfusion Injury
Thoracic Surgery
Reperfusion
Free Radicals
Myocardium
Peptide Hydrolases
Oxygen

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Effect of myocardial ischaemia-reperfusion on granulocyte elastase release. / Ceriana, P.

In: Anaesthesia and Intensive Care, Vol. 20, No. 2, 1992, p. 187-190.

Research output: Contribution to journalArticle

@article{25d21fb474dd4900bbdcf2c8f2a1a5f5,
title = "Effect of myocardial ischaemia-reperfusion on granulocyte elastase release",
abstract = "During extracorporeal circulation, activation and degranulation of neutrophil granulocytes occur, with subsequent massive release of elastase, a neutral protease stored in the azurophil granules. Neutrophil granulocytes and oxygen-derived free radicals are intimately involved in the occurrence of reperfusion injury, which affects especially the lungs and the myocardium. In this study we evaluated changes in the leucocyte count and of the plasma elastase levels in the coronary circulation during open heart surgery. We collected blood samples from the radial artery and the coronary sinus before skin incision, after onset of cardiopulmonary bypass and 5, 15, 30, 60, 120 minutes after the release of the aortic cross-clamp. The leucocyte count did not change significantly during the operation and no difference in leucocyte count was found between the radial artery and the coronary sinus. Plasma elastase levels significantly increased after the onset of cardiopulmonary bypass (P = 0.007) and after the release of the aortic cross-clamp (P = 0.001). Moreover, significantly higher values were found in the coronary sinus than in the radial artery 5 (P = 0.04), 15 (P = 0.02) and 30 (P = 0.02) minutes after the release of the aortic cross-clamp. We assume, therefore, that during the early phase of reperfusion, degranulation of neutrophil granulocytes occurs in the coronary circulation.",
author = "P. Ceriana",
year = "1992",
language = "English",
volume = "20",
pages = "187--190",
journal = "Anaesthesia and Intensive Care",
issn = "0310-057X",
publisher = "Australian Society of Anaesthetists",
number = "2",

}

TY - JOUR

T1 - Effect of myocardial ischaemia-reperfusion on granulocyte elastase release

AU - Ceriana, P.

PY - 1992

Y1 - 1992

N2 - During extracorporeal circulation, activation and degranulation of neutrophil granulocytes occur, with subsequent massive release of elastase, a neutral protease stored in the azurophil granules. Neutrophil granulocytes and oxygen-derived free radicals are intimately involved in the occurrence of reperfusion injury, which affects especially the lungs and the myocardium. In this study we evaluated changes in the leucocyte count and of the plasma elastase levels in the coronary circulation during open heart surgery. We collected blood samples from the radial artery and the coronary sinus before skin incision, after onset of cardiopulmonary bypass and 5, 15, 30, 60, 120 minutes after the release of the aortic cross-clamp. The leucocyte count did not change significantly during the operation and no difference in leucocyte count was found between the radial artery and the coronary sinus. Plasma elastase levels significantly increased after the onset of cardiopulmonary bypass (P = 0.007) and after the release of the aortic cross-clamp (P = 0.001). Moreover, significantly higher values were found in the coronary sinus than in the radial artery 5 (P = 0.04), 15 (P = 0.02) and 30 (P = 0.02) minutes after the release of the aortic cross-clamp. We assume, therefore, that during the early phase of reperfusion, degranulation of neutrophil granulocytes occurs in the coronary circulation.

AB - During extracorporeal circulation, activation and degranulation of neutrophil granulocytes occur, with subsequent massive release of elastase, a neutral protease stored in the azurophil granules. Neutrophil granulocytes and oxygen-derived free radicals are intimately involved in the occurrence of reperfusion injury, which affects especially the lungs and the myocardium. In this study we evaluated changes in the leucocyte count and of the plasma elastase levels in the coronary circulation during open heart surgery. We collected blood samples from the radial artery and the coronary sinus before skin incision, after onset of cardiopulmonary bypass and 5, 15, 30, 60, 120 minutes after the release of the aortic cross-clamp. The leucocyte count did not change significantly during the operation and no difference in leucocyte count was found between the radial artery and the coronary sinus. Plasma elastase levels significantly increased after the onset of cardiopulmonary bypass (P = 0.007) and after the release of the aortic cross-clamp (P = 0.001). Moreover, significantly higher values were found in the coronary sinus than in the radial artery 5 (P = 0.04), 15 (P = 0.02) and 30 (P = 0.02) minutes after the release of the aortic cross-clamp. We assume, therefore, that during the early phase of reperfusion, degranulation of neutrophil granulocytes occurs in the coronary circulation.

UR - http://www.scopus.com/inward/record.url?scp=0026643006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026643006&partnerID=8YFLogxK

M3 - Article

C2 - 1595854

AN - SCOPUS:0026643006

VL - 20

SP - 187

EP - 190

JO - Anaesthesia and Intensive Care

JF - Anaesthesia and Intensive Care

SN - 0310-057X

IS - 2

ER -