Effect of n-3 fatty acid ethyl ester supplementation on fatty acid composition of the single platelet phospholipids and on platelet functions

Domenico Prisco, Monica Filippini, Isa Francalanci, Rita Paniccia, Gian Franco Gensini, Gian Gastone Neri Serneri

Research output: Contribution to journalArticle

Abstract

Twenty healthy male volunteers were randomly assigned to receive either four 1-g capsules of n-3 polyunsaturated fatty acids (PUFA) ethyl esters or four 1-g capsules of olive oil (as placebo) for a period of 4 months, followed by a 3-month wash-out period. Fatty acids of platelet phospholipid fractions, platelet aggregation, and thromboxane B2 (TXB2) formation were analyzed at 0, 2, and 4 months of treatment and at 1, 2, and 3 months of wash-out. During n-3 PUFA supplementation, accumulations of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic (DHA) acids were markedly increased after 2 months, with slight differences in further accumulation up to 4 months among the various phospholipid fractions. Significant decreases in platelet sensitivity to collagen, serum TXB2 levels, and urinary TXB2 metabolites were also observed following n-3 PUFA treatment. During the first and second month of wash-out, slight differences were observed in changes of various fatty acids among different phospholipid fractions, but after 3 months of wash-out, alterations were no longer detectable with respect to pretreatment values. After 3 months of wash-out, platelet function parameters also were returned to baseline. Thus, both platelet lipids and function are influenced by n-3 PUFA ethyl ester supplementation, and significant alterations are still detectable after 2 months of wash-out.

Original languageEnglish
Pages (from-to)562-569
Number of pages8
JournalMetabolism
Volume44
Issue number5
DOIs
Publication statusPublished - 1995

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Omega-3 Fatty Acids
Thromboxane B2
Phospholipids
Esters
Fatty Acids
Blood Platelets
Capsules
Eicosapentaenoic Acid
Docosahexaenoic Acids
Platelet Aggregation
Healthy Volunteers
Collagen
Placebos
Lipids
Therapeutics
Serum

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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Effect of n-3 fatty acid ethyl ester supplementation on fatty acid composition of the single platelet phospholipids and on platelet functions. / Prisco, Domenico; Filippini, Monica; Francalanci, Isa; Paniccia, Rita; Gensini, Gian Franco; Serneri, Gian Gastone Neri.

In: Metabolism, Vol. 44, No. 5, 1995, p. 562-569.

Research output: Contribution to journalArticle

Prisco, Domenico ; Filippini, Monica ; Francalanci, Isa ; Paniccia, Rita ; Gensini, Gian Franco ; Serneri, Gian Gastone Neri. / Effect of n-3 fatty acid ethyl ester supplementation on fatty acid composition of the single platelet phospholipids and on platelet functions. In: Metabolism. 1995 ; Vol. 44, No. 5. pp. 562-569.
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