Abstract
We evaluated the effect of four non-steroidal drugs on in vitro lymphocyte blastogenesis to the aspecific mitogens phytohaemoagglutinin (PHA), concanavalin A (Con A) and Pokeweed mitogen (PWM). The anti-inflammatory drugs studied were phenylbutazone, indomethacin, lysine acetylsalicylate and ibuprofen lysinate. We used the technique of Penhale et al. for in vitro cultures. The mean percent blastogenic response was nearly 100% or more, for all drugs tested, at the 4 μg/ml and 2 μg/ml concentrations but at the concentrations of 1000 μg/ml and 500 μg/ml, all the drugs tested significantly inhibited lymphocyte blastogenesis. Lymphocyte cultures containing 62.5 μg/ml of indomethacin, lysine acetylsalicylate (=34.7 μg/ml ASA) and ibuprofen lysinate (=36.2 μg/ml ibuprofen) reached in most cases response levels close to normal.
| Original language | English |
|---|---|
| Pages (from-to) | 570 |
| Number of pages | 1 |
| Journal | IRCS Medical Science |
| Volume | 7 |
| Issue number | 11 |
| Publication status | Published - 1979 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
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Effect of non-steroidal anti-inflammatory drugs on lymphocyte blastogenesis in vitro. / Corsini, G.; Puppo, F.; Zavarise, G. M.
In: IRCS Medical Science, Vol. 7, No. 11, 1979, p. 570.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of non-steroidal anti-inflammatory drugs on lymphocyte blastogenesis in vitro
AU - Corsini, G.
AU - Puppo, F.
AU - Zavarise, G. M.
PY - 1979
Y1 - 1979
N2 - We evaluated the effect of four non-steroidal drugs on in vitro lymphocyte blastogenesis to the aspecific mitogens phytohaemoagglutinin (PHA), concanavalin A (Con A) and Pokeweed mitogen (PWM). The anti-inflammatory drugs studied were phenylbutazone, indomethacin, lysine acetylsalicylate and ibuprofen lysinate. We used the technique of Penhale et al. for in vitro cultures. The mean percent blastogenic response was nearly 100% or more, for all drugs tested, at the 4 μg/ml and 2 μg/ml concentrations but at the concentrations of 1000 μg/ml and 500 μg/ml, all the drugs tested significantly inhibited lymphocyte blastogenesis. Lymphocyte cultures containing 62.5 μg/ml of indomethacin, lysine acetylsalicylate (=34.7 μg/ml ASA) and ibuprofen lysinate (=36.2 μg/ml ibuprofen) reached in most cases response levels close to normal.
AB - We evaluated the effect of four non-steroidal drugs on in vitro lymphocyte blastogenesis to the aspecific mitogens phytohaemoagglutinin (PHA), concanavalin A (Con A) and Pokeweed mitogen (PWM). The anti-inflammatory drugs studied were phenylbutazone, indomethacin, lysine acetylsalicylate and ibuprofen lysinate. We used the technique of Penhale et al. for in vitro cultures. The mean percent blastogenic response was nearly 100% or more, for all drugs tested, at the 4 μg/ml and 2 μg/ml concentrations but at the concentrations of 1000 μg/ml and 500 μg/ml, all the drugs tested significantly inhibited lymphocyte blastogenesis. Lymphocyte cultures containing 62.5 μg/ml of indomethacin, lysine acetylsalicylate (=34.7 μg/ml ASA) and ibuprofen lysinate (=36.2 μg/ml ibuprofen) reached in most cases response levels close to normal.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018287319&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0018287319
VL - 7
SP - 570
JO - IRCS Medical Science
JF - IRCS Medical Science
SN - 0305-6651
IS - 11
ER -