Effect of oligomer length and base substitutions on the cytotoxic activity and specific nuclear protein recognition of GTn oligonucleotides in the human leukemic CCRF-CEM cell line

C Morassutti, B Dapas, B Scaggiante, G Paroni, L Xodo, F Quadrifoglio

Research output: Contribution to journalArticle

Abstract

We have identified phosphodiester oligonucleotides composed of G and T bases, named GTn, which are able to inhibit the cellular growth of human cancer cell lines by recognising specific nuclear proteins. We demonstrated that GTn oligonucleotides require a length of at least 20 nucleotides in order to exert a significant cytotoxic effect and to retain the specific protein binding ability. In addition, we found that GTn cytotoxicity was lost when A or C bases were introduced at either 3' and 5' end or within the GTn sequences.

Original languageEnglish
Pages (from-to)1711-6
Number of pages6
JournalNucleosides and Nucleotides
Volume18
Issue number6-7
Publication statusPublished - Sep 4 1999

Keywords

  • Biopolymers
  • Humans
  • Nuclear Proteins/metabolism
  • Oligonucleotides/metabolism
  • Tumor Cells, Cultured

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