Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth: role of ABCB1 and PAI-1 gene polymorphisms.

Sara De Iudicibus, Gabriele Stocco, Gaetano Castronovo, Camillo Pico, Roberto Racano, Massimo Borelli, Lorenzo Bevilacqua, Roberto Di Lenarda, Fiora Bartoli, Giuliana Decorti

Research output: Contribution to journalArticle

Abstract

Etiological periodontal therapy is effective in reducing cyclosporin A-induced gingival overgrowth, but a high variability among subjects has been observed. This study aimed to evaluate the role of polymorphisms in PAI-1 and A BCB1 genes on the course of this side effect following periodontal therapy. Forty-five transplant patients were subjected to nonsurgical periodontal therapy and evaluated for hypertrophy index, probing depths, bleeding, and plaque scores at baseline, and after 3 and 6 months. A BCB1 (C3435T and G2677T) and PAI-1 (4G/5G) polymorphisms were studied with polymerase chain reaction-restriction fragment length polymorphism and allele-specific polymerase chain reaction respectively. All the monitored periodontal indexes decreased significantly during the six months. Modeling of hypertrophy index by linearmixed- effect models (allowing non-normal distribution of the outcome variable hypertrophy index) resulted in the selection as the most significant model, of the one comprising the independent variables: time, C 3435T genotype, and their interaction term. This model indicated that C 3435T-mutated patients had significantly higher baseline hypertrophy index values (90% Markov chain Monte C arlo empirical confidence intervals: 5.08, 30.00). The decrease in hypertrophy index values over time showed a trend toward being faster in mutated than nonmutated patients (interaction time: C 3435T nonmutated, 90% Markov chain Monte C arlo empirical confidence interval: -11.08, -0.40). When hypertrophy index values were normalized, the significance and trend were lost. No effect of the A BCB1 G2677T and PAI-1 4G/5G polymorphisms was observed. These preliminary results suggest that C 3435T polymorphism is a genetic factor that could influence the course of cyclosporin A-induced gingival overgrowth in transplant patients subjected to periodontal therapy.

Original languageEnglish
Pages (from-to)249-260
Number of pages12
JournalQuintessence international (Berlin, Germany : 1985)
Volume44
Issue number3
Publication statusPublished - Mar 2013

Fingerprint

Gingival Overgrowth
Plasminogen Activator Inhibitor 1
Hypertrophy
Cyclosporine
Genes
Markov Chains
Therapeutics
Confidence Intervals
Transplants
Periodontal Index
Polymerase Chain Reaction
Restriction Fragment Length Polymorphisms
Alleles
Genotype
Hemorrhage

ASJC Scopus subject areas

  • Medicine(all)

Cite this

De Iudicibus, S., Stocco, G., Castronovo, G., Pico, C., Racano, R., Borelli, M., ... Decorti, G. (2013). Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth: role of ABCB1 and PAI-1 gene polymorphisms. Quintessence international (Berlin, Germany : 1985), 44(3), 249-260.

Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth : role of ABCB1 and PAI-1 gene polymorphisms. / De Iudicibus, Sara; Stocco, Gabriele; Castronovo, Gaetano; Pico, Camillo; Racano, Roberto; Borelli, Massimo; Bevilacqua, Lorenzo; Di Lenarda, Roberto; Bartoli, Fiora; Decorti, Giuliana.

In: Quintessence international (Berlin, Germany : 1985), Vol. 44, No. 3, 03.2013, p. 249-260.

Research output: Contribution to journalArticle

De Iudicibus, S, Stocco, G, Castronovo, G, Pico, C, Racano, R, Borelli, M, Bevilacqua, L, Di Lenarda, R, Bartoli, F & Decorti, G 2013, 'Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth: role of ABCB1 and PAI-1 gene polymorphisms.', Quintessence international (Berlin, Germany : 1985), vol. 44, no. 3, pp. 249-260.
De Iudicibus, Sara ; Stocco, Gabriele ; Castronovo, Gaetano ; Pico, Camillo ; Racano, Roberto ; Borelli, Massimo ; Bevilacqua, Lorenzo ; Di Lenarda, Roberto ; Bartoli, Fiora ; Decorti, Giuliana. / Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth : role of ABCB1 and PAI-1 gene polymorphisms. In: Quintessence international (Berlin, Germany : 1985). 2013 ; Vol. 44, No. 3. pp. 249-260.
@article{79047d7b7c2e42fcb3ece9753e04646d,
title = "Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth: role of ABCB1 and PAI-1 gene polymorphisms.",
abstract = "Etiological periodontal therapy is effective in reducing cyclosporin A-induced gingival overgrowth, but a high variability among subjects has been observed. This study aimed to evaluate the role of polymorphisms in PAI-1 and A BCB1 genes on the course of this side effect following periodontal therapy. Forty-five transplant patients were subjected to nonsurgical periodontal therapy and evaluated for hypertrophy index, probing depths, bleeding, and plaque scores at baseline, and after 3 and 6 months. A BCB1 (C3435T and G2677T) and PAI-1 (4G/5G) polymorphisms were studied with polymerase chain reaction-restriction fragment length polymorphism and allele-specific polymerase chain reaction respectively. All the monitored periodontal indexes decreased significantly during the six months. Modeling of hypertrophy index by linearmixed- effect models (allowing non-normal distribution of the outcome variable hypertrophy index) resulted in the selection as the most significant model, of the one comprising the independent variables: time, C 3435T genotype, and their interaction term. This model indicated that C 3435T-mutated patients had significantly higher baseline hypertrophy index values (90{\%} Markov chain Monte C arlo empirical confidence intervals: 5.08, 30.00). The decrease in hypertrophy index values over time showed a trend toward being faster in mutated than nonmutated patients (interaction time: C 3435T nonmutated, 90{\%} Markov chain Monte C arlo empirical confidence interval: -11.08, -0.40). When hypertrophy index values were normalized, the significance and trend were lost. No effect of the A BCB1 G2677T and PAI-1 4G/5G polymorphisms was observed. These preliminary results suggest that C 3435T polymorphism is a genetic factor that could influence the course of cyclosporin A-induced gingival overgrowth in transplant patients subjected to periodontal therapy.",
author = "{De Iudicibus}, Sara and Gabriele Stocco and Gaetano Castronovo and Camillo Pico and Roberto Racano and Massimo Borelli and Lorenzo Bevilacqua and {Di Lenarda}, Roberto and Fiora Bartoli and Giuliana Decorti",
year = "2013",
month = "3",
language = "English",
volume = "44",
pages = "249--260",
journal = "Quintessence International",
issn = "0033-6572",
publisher = "Quintessence Publishing Company",
number = "3",

}

TY - JOUR

T1 - Effect of periodontal therapy on the course of cyclosporin-induced gingival overgrowth

T2 - role of ABCB1 and PAI-1 gene polymorphisms.

AU - De Iudicibus, Sara

AU - Stocco, Gabriele

AU - Castronovo, Gaetano

AU - Pico, Camillo

AU - Racano, Roberto

AU - Borelli, Massimo

AU - Bevilacqua, Lorenzo

AU - Di Lenarda, Roberto

AU - Bartoli, Fiora

AU - Decorti, Giuliana

PY - 2013/3

Y1 - 2013/3

N2 - Etiological periodontal therapy is effective in reducing cyclosporin A-induced gingival overgrowth, but a high variability among subjects has been observed. This study aimed to evaluate the role of polymorphisms in PAI-1 and A BCB1 genes on the course of this side effect following periodontal therapy. Forty-five transplant patients were subjected to nonsurgical periodontal therapy and evaluated for hypertrophy index, probing depths, bleeding, and plaque scores at baseline, and after 3 and 6 months. A BCB1 (C3435T and G2677T) and PAI-1 (4G/5G) polymorphisms were studied with polymerase chain reaction-restriction fragment length polymorphism and allele-specific polymerase chain reaction respectively. All the monitored periodontal indexes decreased significantly during the six months. Modeling of hypertrophy index by linearmixed- effect models (allowing non-normal distribution of the outcome variable hypertrophy index) resulted in the selection as the most significant model, of the one comprising the independent variables: time, C 3435T genotype, and their interaction term. This model indicated that C 3435T-mutated patients had significantly higher baseline hypertrophy index values (90% Markov chain Monte C arlo empirical confidence intervals: 5.08, 30.00). The decrease in hypertrophy index values over time showed a trend toward being faster in mutated than nonmutated patients (interaction time: C 3435T nonmutated, 90% Markov chain Monte C arlo empirical confidence interval: -11.08, -0.40). When hypertrophy index values were normalized, the significance and trend were lost. No effect of the A BCB1 G2677T and PAI-1 4G/5G polymorphisms was observed. These preliminary results suggest that C 3435T polymorphism is a genetic factor that could influence the course of cyclosporin A-induced gingival overgrowth in transplant patients subjected to periodontal therapy.

AB - Etiological periodontal therapy is effective in reducing cyclosporin A-induced gingival overgrowth, but a high variability among subjects has been observed. This study aimed to evaluate the role of polymorphisms in PAI-1 and A BCB1 genes on the course of this side effect following periodontal therapy. Forty-five transplant patients were subjected to nonsurgical periodontal therapy and evaluated for hypertrophy index, probing depths, bleeding, and plaque scores at baseline, and after 3 and 6 months. A BCB1 (C3435T and G2677T) and PAI-1 (4G/5G) polymorphisms were studied with polymerase chain reaction-restriction fragment length polymorphism and allele-specific polymerase chain reaction respectively. All the monitored periodontal indexes decreased significantly during the six months. Modeling of hypertrophy index by linearmixed- effect models (allowing non-normal distribution of the outcome variable hypertrophy index) resulted in the selection as the most significant model, of the one comprising the independent variables: time, C 3435T genotype, and their interaction term. This model indicated that C 3435T-mutated patients had significantly higher baseline hypertrophy index values (90% Markov chain Monte C arlo empirical confidence intervals: 5.08, 30.00). The decrease in hypertrophy index values over time showed a trend toward being faster in mutated than nonmutated patients (interaction time: C 3435T nonmutated, 90% Markov chain Monte C arlo empirical confidence interval: -11.08, -0.40). When hypertrophy index values were normalized, the significance and trend were lost. No effect of the A BCB1 G2677T and PAI-1 4G/5G polymorphisms was observed. These preliminary results suggest that C 3435T polymorphism is a genetic factor that could influence the course of cyclosporin A-induced gingival overgrowth in transplant patients subjected to periodontal therapy.

UR - http://www.scopus.com/inward/record.url?scp=84892526240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892526240&partnerID=8YFLogxK

M3 - Article

C2 - 23444206

AN - SCOPUS:84892526240

VL - 44

SP - 249

EP - 260

JO - Quintessence International

JF - Quintessence International

SN - 0033-6572

IS - 3

ER -