Effect of PM10 on human spermatozoa: An In Vitro study

M. Geminiani, F. Moretti, R. Cardinali, P. Lattaioli, F. Cetta, C. Castellini, G. Collodel

Research output: Contribution to journalArticlepeer-review

Abstract

This study was aimed at evaluating the in vitro effects of particulate matter (ranging from 2.5-10 μm in an aerodynamic diameter, PM10) from an urban area on the motility of human spermatozoa. Samples of swim up selected human spermatozoa from three healthy men with normal semen parameters, according to WHO guidelines, were incubated for 4 hours with 10, 50 and 75 μg/ml of PM10. Kinetic parameters were evaluated with Computer-Assisted Sperm Analysis (CASA) and the presence of apoptosis and necrosis was assessed by AnnexinV/Propidium iodide assay. Data was statistically elaborated. Incubation of sperm with 10 μg/ml PM10 for 4 hours did not affect sperm motility. On the contrary, a decrease in progressive motility was evident at 50 μg/ml and 75 μg/ml PM10. Among the kinetic parameters assessed with CASA system, the linearity variable was significantly lower in sperm treated with 50 μg/ml and 75 μg/ml PM10 vs controls (P

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalOpen Andrology Journal
Volume3
DOIs
Publication statusPublished - 2011

Keywords

  • CASA
  • Human sperm motility
  • PM10
  • Sperm necrosis

ASJC Scopus subject areas

  • Reproductive Medicine

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