Abstract
Poly(ethylene glycol) (PEG) stabilized liposomes are widely used in drug delivery research because these surface grafted polymers stabilize liposomes in blood stream. However, hydrophilic polymers also affect lipid membrane organization and it is fundamental to investigate their effect on the physicochemical properties of lipid bilayers as well as on their interaction with biological membrane. PEG monolaurate was included in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and one of two cationic gemini surfactants that differ each other for the configuration of a stereogenic center on the polar headgroup. These formulations previously showed good efficiency as drug and DNA delivery systems. The effect of the presence of different amounts of a PEG-lipid and of the liposome preparation protocol was investigated by turbidimetry, dynamic laser light scattering, differential scanning calorimetry and electrophoretic mobility. Flow cytometry and laser scanning confocal microscopy on murine (C6) and on human glioblastoma (LN229) cell lines were also carried out to evaluate the influence of pegylation and of the protocol of preparation on the interaction of liposomes with the biological milieu.
| Original language | English |
|---|---|
| Pages (from-to) | 444-450 |
| Number of pages | 7 |
| Journal | Colloids and Surfaces A: Physicochemical and Engineering Aspects |
| Volume | 532 |
| DOIs | |
| Publication status | Published - Nov 5 2017 |
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Keywords
- Intracellular uptake and distribution.
- Pegylated liposomes
- Physicochemical properties
- Preparation protocol
- Stereochemistry
ASJC Scopus subject areas
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Effect of preparation protocol on physicochemical features and biointeractions of pegylated liposomes. / Giansanti, Luisa; Bozzuto, Giuseppina; Fracassi, Alessandro; Bombelli, Cecilia; Stringaro, Annarita; Molinari, Agnese; Piozzi, Antonella; Sennato, Simona; Mancini, Giovanna.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 532, 05.11.2017, p. 444-450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of preparation protocol on physicochemical features and biointeractions of pegylated liposomes
AU - Giansanti, Luisa
AU - Bozzuto, Giuseppina
AU - Fracassi, Alessandro
AU - Bombelli, Cecilia
AU - Stringaro, Annarita
AU - Molinari, Agnese
AU - Piozzi, Antonella
AU - Sennato, Simona
AU - Mancini, Giovanna
PY - 2017/11/5
Y1 - 2017/11/5
N2 - Poly(ethylene glycol) (PEG) stabilized liposomes are widely used in drug delivery research because these surface grafted polymers stabilize liposomes in blood stream. However, hydrophilic polymers also affect lipid membrane organization and it is fundamental to investigate their effect on the physicochemical properties of lipid bilayers as well as on their interaction with biological membrane. PEG monolaurate was included in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and one of two cationic gemini surfactants that differ each other for the configuration of a stereogenic center on the polar headgroup. These formulations previously showed good efficiency as drug and DNA delivery systems. The effect of the presence of different amounts of a PEG-lipid and of the liposome preparation protocol was investigated by turbidimetry, dynamic laser light scattering, differential scanning calorimetry and electrophoretic mobility. Flow cytometry and laser scanning confocal microscopy on murine (C6) and on human glioblastoma (LN229) cell lines were also carried out to evaluate the influence of pegylation and of the protocol of preparation on the interaction of liposomes with the biological milieu.
AB - Poly(ethylene glycol) (PEG) stabilized liposomes are widely used in drug delivery research because these surface grafted polymers stabilize liposomes in blood stream. However, hydrophilic polymers also affect lipid membrane organization and it is fundamental to investigate their effect on the physicochemical properties of lipid bilayers as well as on their interaction with biological membrane. PEG monolaurate was included in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and one of two cationic gemini surfactants that differ each other for the configuration of a stereogenic center on the polar headgroup. These formulations previously showed good efficiency as drug and DNA delivery systems. The effect of the presence of different amounts of a PEG-lipid and of the liposome preparation protocol was investigated by turbidimetry, dynamic laser light scattering, differential scanning calorimetry and electrophoretic mobility. Flow cytometry and laser scanning confocal microscopy on murine (C6) and on human glioblastoma (LN229) cell lines were also carried out to evaluate the influence of pegylation and of the protocol of preparation on the interaction of liposomes with the biological milieu.
KW - Intracellular uptake and distribution.
KW - Pegylated liposomes
KW - Physicochemical properties
KW - Preparation protocol
KW - Stereochemistry
UR - http://www.scopus.com/inward/record.url?scp=85018372261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018372261&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2017.04.037
DO - 10.1016/j.colsurfa.2017.04.037
M3 - Article
AN - SCOPUS:85018372261
VL - 532
SP - 444
EP - 450
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
SN - 0927-7757
ER -