Effect of remote ischemic preconditioning on platelet activation and reactivity induced by ablation for atrial fibrillation

Alessandra Stazi, Giancarla Scalone, Marianna Laurito, Maria Milo, Gemma Pelargonio, Maria Lucia Narducci, Rossella Parrinello, Stefano Figliozzi, Gianluigi Bencardino, Francesco Perna, Gaetano A. Lanza, Filippo Crea

Research output: Contribution to journalArticlepeer-review


BACKGROUND-: Radiofrequency ablation of atrial fibrillation has been associated with some risk of thromboembolic events. Previous studies showed that preventive short episodes of forearm ischemia (remote ischemic preconditioning [IPC]) reduce exercise-induced platelet reactivity. In this study, we assessed whether remote IPC has any effect on platelet activation induced by radiofrequency ablation of atrial fibrillation. METHODS AND RESULTS-: We randomized 19 patients (age, 54.7±11 years; 17 male) undergoing radiofrequency catheter ablation of paroxysmal atrial fibrillation to receive remote IPC or sham intermittent forearm ischemia (control subjects) before the procedure. Blood venous samples were collected before and after remote IPC/sham ischemia, at the end of the ablation procedure, and 24 hours later. Platelet activation and reactivity were assessed by flow cytometry by measuring monocyte-platelet aggregate formation, platelet CD41 in the monocyte-platelet aggregate gate, and platelet CD41 and CD62 in the platelet gate in the absence and presence of ADP stimulation. At baseline, there were no differences between groups in platelet variables. Radiofrequency ablation induced platelet activation in both groups, which persisted after 24 hours. However, compared with control subjects, remote IPC patients showed a lower increase in all platelet variables, including monocyte-platelet aggregate formation (P

Original languageEnglish
Pages (from-to)11-17
Number of pages7
Issue number1
Publication statusPublished - 2014


  • atrial fibrillation
  • blood platelets
  • catheter ablation
  • ischemic preconditioning
  • platelet activation.

ASJC Scopus subject areas

  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine


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