TY - JOUR
T1 - Effect of remote ischemic preconditioning on platelet activation and reactivity induced by ablation for atrial fibrillation
AU - Stazi, Alessandra
AU - Scalone, Giancarla
AU - Laurito, Marianna
AU - Milo, Maria
AU - Pelargonio, Gemma
AU - Narducci, Maria Lucia
AU - Parrinello, Rossella
AU - Figliozzi, Stefano
AU - Bencardino, Gianluigi
AU - Perna, Francesco
AU - Lanza, Gaetano A.
AU - Crea, Filippo
PY - 2014
Y1 - 2014
N2 - BACKGROUND-: Radiofrequency ablation of atrial fibrillation has been associated with some risk of thromboembolic events. Previous studies showed that preventive short episodes of forearm ischemia (remote ischemic preconditioning [IPC]) reduce exercise-induced platelet reactivity. In this study, we assessed whether remote IPC has any effect on platelet activation induced by radiofrequency ablation of atrial fibrillation. METHODS AND RESULTS-: We randomized 19 patients (age, 54.7±11 years; 17 male) undergoing radiofrequency catheter ablation of paroxysmal atrial fibrillation to receive remote IPC or sham intermittent forearm ischemia (control subjects) before the procedure. Blood venous samples were collected before and after remote IPC/sham ischemia, at the end of the ablation procedure, and 24 hours later. Platelet activation and reactivity were assessed by flow cytometry by measuring monocyte-platelet aggregate formation, platelet CD41 in the monocyte-platelet aggregate gate, and platelet CD41 and CD62 in the platelet gate in the absence and presence of ADP stimulation. At baseline, there were no differences between groups in platelet variables. Radiofrequency ablation induced platelet activation in both groups, which persisted after 24 hours. However, compared with control subjects, remote IPC patients showed a lower increase in all platelet variables, including monocyte-platelet aggregate formation (P
AB - BACKGROUND-: Radiofrequency ablation of atrial fibrillation has been associated with some risk of thromboembolic events. Previous studies showed that preventive short episodes of forearm ischemia (remote ischemic preconditioning [IPC]) reduce exercise-induced platelet reactivity. In this study, we assessed whether remote IPC has any effect on platelet activation induced by radiofrequency ablation of atrial fibrillation. METHODS AND RESULTS-: We randomized 19 patients (age, 54.7±11 years; 17 male) undergoing radiofrequency catheter ablation of paroxysmal atrial fibrillation to receive remote IPC or sham intermittent forearm ischemia (control subjects) before the procedure. Blood venous samples were collected before and after remote IPC/sham ischemia, at the end of the ablation procedure, and 24 hours later. Platelet activation and reactivity were assessed by flow cytometry by measuring monocyte-platelet aggregate formation, platelet CD41 in the monocyte-platelet aggregate gate, and platelet CD41 and CD62 in the platelet gate in the absence and presence of ADP stimulation. At baseline, there were no differences between groups in platelet variables. Radiofrequency ablation induced platelet activation in both groups, which persisted after 24 hours. However, compared with control subjects, remote IPC patients showed a lower increase in all platelet variables, including monocyte-platelet aggregate formation (P
KW - atrial fibrillation
KW - blood platelets
KW - catheter ablation
KW - ischemic preconditioning
KW - platelet activation.
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U2 - 10.1161/CIRCULATIONAHA.113.005336
DO - 10.1161/CIRCULATIONAHA.113.005336
M3 - Article
C2 - 24277055
AN - SCOPUS:84891861654
VL - 129
SP - 11
EP - 17
JO - Circulation
JF - Circulation
SN - 0009-7322
IS - 1
ER -