We have investigated the properties of dopamine-dependent adenylate cyclase in rat striatal homogenates, 20 h after reserpine treatment. In this experimental condition, we have found that the affinity of the enzyme for dopamine is greatly enhanced. On the other hand, the concentration of apomorphine required to produced half-maximal activation of the enzyme in striatal homogenates of controls and reserpine-treated rats is not changed. The unchanged affinity of adenylate cyclase for the substrate (ATP:Mg++) indicates that reserpine probably affects the receptor component of the enzyme.
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