Effect of rMnSOD on Sodium Reabsorption in Renal Proximal Tubule in Ochratoxin A-Treated Rats

Sara Damiano, Maria V Puzio, Caterina Squillacioti, Nicola Mirabella, Enrica Zona, Aldo Mancini, Antonella Borrelli, Carlo Astarita, Silvia Boffo, Antonio Giordano, Luigi Avallone, Salvatore Florio, Roberto Ciarcia

Research output: Contribution to journalArticle

Abstract

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium that represent toxic real threat for human beings and animal health. In this study we evaluated the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) on oxidative stress and on the alterations of fluid reabsorption in renal proximal tubule (PT) as possible causes of OTA nephrotoxicity. Finally, we have measured the concentration of O2- in the kidney through dihydroethidium assay (DHE) and nitric oxide (NO) concentration through nitrites and nitrates assay. Male Sprague Dawley rats weighing 120-150 g were treated for 14 days by gavage, as follows: Control group, 12 rats received a corresponding amount of saline solution (including 10% DMSO); rMnSOD group, 12 rats treated with rMnSOD (10 µg/kg bw); OTA group, 12 rats treated with OTA (0.5 mg/kg bw) dissolved in 10% DMSO and then scaled to required volume with corn oil; rMnSOD + OTA, 12 rats treated with rMnSOD (10 µg/kg bw) plus OTA (0.5 mg/kg bw). Our results have shown that rMnSOD restores the alteration of reabsorption in PT in rats treated with OTA plus rMnSOD, probably through the response to pressure natriuresis, where nitric oxide plays a key role. Moreover, rMnSOD prevents the nephrotoxicity induced by OTA probably restoring the balance between superoxide and NO that is most probably the cause of hypertension and renal functional alterations through the inhibition of NO synthase. In conclusion these data provide important information for understanding of mechanism of toxic action of OTA. J. Cell. Biochem. 119: 424-430, 2018. © 2017 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)424-430
Number of pages7
JournalJournal of Cellular Biochemistry
Volume119
Issue number1
DOIs
Publication statusPublished - Jan 2018

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Proximal Kidney Tubule
Rats
Sodium
Nitric Oxide
Dimethyl Sulfoxide
Assays
Toxic Actions
ochratoxin A
Natriuresis
Renal Hypertension
Oxidative stress
Corn Oil
Mycotoxins
Aspergillus
Poisons
Penicillium
Weighing
Manganese
Nitrites
Sodium Chloride

Keywords

  • Journal Article

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Damiano, S., Puzio, M. V., Squillacioti, C., Mirabella, N., Zona, E., Mancini, A., ... Ciarcia, R. (2018). Effect of rMnSOD on Sodium Reabsorption in Renal Proximal Tubule in Ochratoxin A-Treated Rats. Journal of Cellular Biochemistry, 119(1), 424-430. https://doi.org/10.1002/jcb.26197

Effect of rMnSOD on Sodium Reabsorption in Renal Proximal Tubule in Ochratoxin A-Treated Rats. / Damiano, Sara; Puzio, Maria V; Squillacioti, Caterina; Mirabella, Nicola; Zona, Enrica; Mancini, Aldo; Borrelli, Antonella; Astarita, Carlo; Boffo, Silvia; Giordano, Antonio; Avallone, Luigi; Florio, Salvatore; Ciarcia, Roberto.

In: Journal of Cellular Biochemistry, Vol. 119, No. 1, 01.2018, p. 424-430.

Research output: Contribution to journalArticle

Damiano, S, Puzio, MV, Squillacioti, C, Mirabella, N, Zona, E, Mancini, A, Borrelli, A, Astarita, C, Boffo, S, Giordano, A, Avallone, L, Florio, S & Ciarcia, R 2018, 'Effect of rMnSOD on Sodium Reabsorption in Renal Proximal Tubule in Ochratoxin A-Treated Rats', Journal of Cellular Biochemistry, vol. 119, no. 1, pp. 424-430. https://doi.org/10.1002/jcb.26197
Damiano, Sara ; Puzio, Maria V ; Squillacioti, Caterina ; Mirabella, Nicola ; Zona, Enrica ; Mancini, Aldo ; Borrelli, Antonella ; Astarita, Carlo ; Boffo, Silvia ; Giordano, Antonio ; Avallone, Luigi ; Florio, Salvatore ; Ciarcia, Roberto. / Effect of rMnSOD on Sodium Reabsorption in Renal Proximal Tubule in Ochratoxin A-Treated Rats. In: Journal of Cellular Biochemistry. 2018 ; Vol. 119, No. 1. pp. 424-430.
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abstract = "Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium that represent toxic real threat for human beings and animal health. In this study we evaluated the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) on oxidative stress and on the alterations of fluid reabsorption in renal proximal tubule (PT) as possible causes of OTA nephrotoxicity. Finally, we have measured the concentration of O2- in the kidney through dihydroethidium assay (DHE) and nitric oxide (NO) concentration through nitrites and nitrates assay. Male Sprague Dawley rats weighing 120-150 g were treated for 14 days by gavage, as follows: Control group, 12 rats received a corresponding amount of saline solution (including 10{\%} DMSO); rMnSOD group, 12 rats treated with rMnSOD (10 µg/kg bw); OTA group, 12 rats treated with OTA (0.5 mg/kg bw) dissolved in 10{\%} DMSO and then scaled to required volume with corn oil; rMnSOD + OTA, 12 rats treated with rMnSOD (10 µg/kg bw) plus OTA (0.5 mg/kg bw). Our results have shown that rMnSOD restores the alteration of reabsorption in PT in rats treated with OTA plus rMnSOD, probably through the response to pressure natriuresis, where nitric oxide plays a key role. Moreover, rMnSOD prevents the nephrotoxicity induced by OTA probably restoring the balance between superoxide and NO that is most probably the cause of hypertension and renal functional alterations through the inhibition of NO synthase. In conclusion these data provide important information for understanding of mechanism of toxic action of OTA. J. Cell. Biochem. 119: 424-430, 2018. {\circledC} 2017 Wiley Periodicals, Inc.",
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AU - Mirabella, Nicola

AU - Zona, Enrica

AU - Mancini, Aldo

AU - Borrelli, Antonella

AU - Astarita, Carlo

AU - Boffo, Silvia

AU - Giordano, Antonio

AU - Avallone, Luigi

AU - Florio, Salvatore

AU - Ciarcia, Roberto

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N2 - Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium that represent toxic real threat for human beings and animal health. In this study we evaluated the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) on oxidative stress and on the alterations of fluid reabsorption in renal proximal tubule (PT) as possible causes of OTA nephrotoxicity. Finally, we have measured the concentration of O2- in the kidney through dihydroethidium assay (DHE) and nitric oxide (NO) concentration through nitrites and nitrates assay. Male Sprague Dawley rats weighing 120-150 g were treated for 14 days by gavage, as follows: Control group, 12 rats received a corresponding amount of saline solution (including 10% DMSO); rMnSOD group, 12 rats treated with rMnSOD (10 µg/kg bw); OTA group, 12 rats treated with OTA (0.5 mg/kg bw) dissolved in 10% DMSO and then scaled to required volume with corn oil; rMnSOD + OTA, 12 rats treated with rMnSOD (10 µg/kg bw) plus OTA (0.5 mg/kg bw). Our results have shown that rMnSOD restores the alteration of reabsorption in PT in rats treated with OTA plus rMnSOD, probably through the response to pressure natriuresis, where nitric oxide plays a key role. Moreover, rMnSOD prevents the nephrotoxicity induced by OTA probably restoring the balance between superoxide and NO that is most probably the cause of hypertension and renal functional alterations through the inhibition of NO synthase. In conclusion these data provide important information for understanding of mechanism of toxic action of OTA. J. Cell. Biochem. 119: 424-430, 2018. © 2017 Wiley Periodicals, Inc.

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