Effect of S. cerevisiae APN1 protein on mammalian DNA base excision repair

Massimo Bogliolo, Enrico Cappelli, Andrea D'Osualdo, Ottavio Rossi, Ottavia Barbieri, Mark R. Kelley, Guido Frosina

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mammalian cells transfected with the S. cerevisiae APN1 protein acquire resistance to oxidizing agents, the damage of which are mainly repaired via DNA base excision repair (BER). We have recently hypothesized that this effect might be linked to the possible capacity of APN1 to accelerate mammalian BER by its 3′ diesterase activity. We have investigated here the effect of pure APN1 protein on BER performed by mouse embryonic fibroblast extracts. No significant acceleration was observed in the repair of either a single AP site cleaved by the bifunctional glycosylase NTH of E.coli or the repair of a single 8-oxoguanine, initiated by the bifunctional glycosylase OGG1. Similarly, no significant effect was observed on the repair of a single U (initiated by the monofunctional glycosylase U DNA glycosylase) or the repair of a single natural abasic site. The inability of APN1 to increase the efficiency of BER initiated by bifunctional glycosylases indicates that removal of 3′ blocking fragments is not the rate limiting step of this repair pathway.

Original languageEnglish
Pages (from-to)2797-2804
Number of pages8
JournalAnticancer Research
Volume22
Issue number5
Publication statusPublished - Sep 2002

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Saccharomyces cerevisiae Proteins
DNA Repair
DNA
DNA Glycosylases
Oxidants
Fibroblasts
Escherichia coli
Proteins

Keywords

  • APN1 protein
  • DNA base excision repair
  • Glycosylase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bogliolo, M., Cappelli, E., D'Osualdo, A., Rossi, O., Barbieri, O., Kelley, M. R., & Frosina, G. (2002). Effect of S. cerevisiae APN1 protein on mammalian DNA base excision repair. Anticancer Research, 22(5), 2797-2804.

Effect of S. cerevisiae APN1 protein on mammalian DNA base excision repair. / Bogliolo, Massimo; Cappelli, Enrico; D'Osualdo, Andrea; Rossi, Ottavio; Barbieri, Ottavia; Kelley, Mark R.; Frosina, Guido.

In: Anticancer Research, Vol. 22, No. 5, 09.2002, p. 2797-2804.

Research output: Contribution to journalArticle

Bogliolo, M, Cappelli, E, D'Osualdo, A, Rossi, O, Barbieri, O, Kelley, MR & Frosina, G 2002, 'Effect of S. cerevisiae APN1 protein on mammalian DNA base excision repair', Anticancer Research, vol. 22, no. 5, pp. 2797-2804.
Bogliolo M, Cappelli E, D'Osualdo A, Rossi O, Barbieri O, Kelley MR et al. Effect of S. cerevisiae APN1 protein on mammalian DNA base excision repair. Anticancer Research. 2002 Sep;22(5):2797-2804.
Bogliolo, Massimo ; Cappelli, Enrico ; D'Osualdo, Andrea ; Rossi, Ottavio ; Barbieri, Ottavia ; Kelley, Mark R. ; Frosina, Guido. / Effect of S. cerevisiae APN1 protein on mammalian DNA base excision repair. In: Anticancer Research. 2002 ; Vol. 22, No. 5. pp. 2797-2804.
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