Effect of Serenoa repens (Permixon®) on the expression of inflammation-related genes: Analysis in primary cell cultures of human prostate carcinoma

Ida Silvestri, Susanna Cattarino, Annamaria Aglianò, Chiara Nicolazzo, Susanna Scarpa, Stefano Salciccia, Luigi Frati, Vincenzo Gentile, Alessandro Sciarra

Research output: Contribution to journalArticlepeer-review

Abstract

Background: To analyze the expression at basal level of inflammation-related cytokines and chemokines and the activation status of the NF-κB pathway, together with the proliferation and apoptosis indexes in two widely used in vitro tumor models, the androgen-dependent human Prostate Cancer (PC) cell line LNCaP and the androgen-independent PC3, and in primary cultures of human PC cells. To assess in these models and primary cultures, the effects of Serenoa repens (LSESr, Permixon®) on proliferation/apoptosis ratio, inflammation-related genes expression and NF-κB pathway activation. Methods. The expression of IL-6, CCL-5, CCL-2, COX-1, COX-2, iNOS inflammation-related genes has been evaluated at the mRNA level in two in vitro human PC models (LNCaP and PC3 cell lines) and in 40 independent human prostatic primary cultures obtained from PC patients undergoing radical prostatectomy. Tissue fragments were collected from both PC lesions and normal hyperplastic tissue counterparts for each case. All cultures were treated with two different amounts of Permixon® (44 and 88 μg/ml) for different time points (16, 24, 48 and 72 hours), depending on the cell type and the assay; the expression of inflammation-related genes, cell growth (proliferation/apoptosis ratio) and NF-κB activation has been analyzed in treated and untreated cells by means of semi-quantitative RNA-PCR, cell proliferation and immunofluorescence respectively. Results: We detected a significant reduction (p

Original languageEnglish
Article number11
JournalJournal of Inflammation (United Kingdom)
Volume10
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Apoptosis
  • Caspase detection
  • Cell cultures
  • Inflammation
  • Proliferation
  • Prostate neoplasm

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry

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