Abstract
Background: To analyze the expression at basal level of inflammation-related cytokines and chemokines and the activation status of the NF-κB pathway, together with the proliferation and apoptosis indexes in two widely used in vitro tumor models, the androgen-dependent human Prostate Cancer (PC) cell line LNCaP and the androgen-independent PC3, and in primary cultures of human PC cells. To assess in these models and primary cultures, the effects of Serenoa repens (LSESr, Permixon®) on proliferation/apoptosis ratio, inflammation-related genes expression and NF-κB pathway activation. Methods. The expression of IL-6, CCL-5, CCL-2, COX-1, COX-2, iNOS inflammation-related genes has been evaluated at the mRNA level in two in vitro human PC models (LNCaP and PC3 cell lines) and in 40 independent human prostatic primary cultures obtained from PC patients undergoing radical prostatectomy. Tissue fragments were collected from both PC lesions and normal hyperplastic tissue counterparts for each case. All cultures were treated with two different amounts of Permixon® (44 and 88 μg/ml) for different time points (16, 24, 48 and 72 hours), depending on the cell type and the assay; the expression of inflammation-related genes, cell growth (proliferation/apoptosis ratio) and NF-κB activation has been analyzed in treated and untreated cells by means of semi-quantitative RNA-PCR, cell proliferation and immunofluorescence respectively. Results: We detected a significant reduction (p
| Original language | English |
|---|---|
| Article number | 11 |
| Journal | Journal of Inflammation (United Kingdom) |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- Apoptosis
- Caspase detection
- Cell cultures
- Inflammation
- Proliferation
- Prostate neoplasm
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
Cite this
Effect of Serenoa repens (Permixon®) on the expression of inflammation-related genes : Analysis in primary cell cultures of human prostate carcinoma. / Silvestri, Ida; Cattarino, Susanna; Aglianò, Annamaria; Nicolazzo, Chiara; Scarpa, Susanna; Salciccia, Stefano; Frati, Luigi; Gentile, Vincenzo; Sciarra, Alessandro.
In: Journal of Inflammation (United Kingdom), Vol. 10, No. 1, 11, 2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Serenoa repens (Permixon®) on the expression of inflammation-related genes
T2 - Analysis in primary cell cultures of human prostate carcinoma
AU - Silvestri, Ida
AU - Cattarino, Susanna
AU - Aglianò, Annamaria
AU - Nicolazzo, Chiara
AU - Scarpa, Susanna
AU - Salciccia, Stefano
AU - Frati, Luigi
AU - Gentile, Vincenzo
AU - Sciarra, Alessandro
PY - 2013
Y1 - 2013
N2 - Background: To analyze the expression at basal level of inflammation-related cytokines and chemokines and the activation status of the NF-κB pathway, together with the proliferation and apoptosis indexes in two widely used in vitro tumor models, the androgen-dependent human Prostate Cancer (PC) cell line LNCaP and the androgen-independent PC3, and in primary cultures of human PC cells. To assess in these models and primary cultures, the effects of Serenoa repens (LSESr, Permixon®) on proliferation/apoptosis ratio, inflammation-related genes expression and NF-κB pathway activation. Methods. The expression of IL-6, CCL-5, CCL-2, COX-1, COX-2, iNOS inflammation-related genes has been evaluated at the mRNA level in two in vitro human PC models (LNCaP and PC3 cell lines) and in 40 independent human prostatic primary cultures obtained from PC patients undergoing radical prostatectomy. Tissue fragments were collected from both PC lesions and normal hyperplastic tissue counterparts for each case. All cultures were treated with two different amounts of Permixon® (44 and 88 μg/ml) for different time points (16, 24, 48 and 72 hours), depending on the cell type and the assay; the expression of inflammation-related genes, cell growth (proliferation/apoptosis ratio) and NF-κB activation has been analyzed in treated and untreated cells by means of semi-quantitative RNA-PCR, cell proliferation and immunofluorescence respectively. Results: We detected a significant reduction (p
AB - Background: To analyze the expression at basal level of inflammation-related cytokines and chemokines and the activation status of the NF-κB pathway, together with the proliferation and apoptosis indexes in two widely used in vitro tumor models, the androgen-dependent human Prostate Cancer (PC) cell line LNCaP and the androgen-independent PC3, and in primary cultures of human PC cells. To assess in these models and primary cultures, the effects of Serenoa repens (LSESr, Permixon®) on proliferation/apoptosis ratio, inflammation-related genes expression and NF-κB pathway activation. Methods. The expression of IL-6, CCL-5, CCL-2, COX-1, COX-2, iNOS inflammation-related genes has been evaluated at the mRNA level in two in vitro human PC models (LNCaP and PC3 cell lines) and in 40 independent human prostatic primary cultures obtained from PC patients undergoing radical prostatectomy. Tissue fragments were collected from both PC lesions and normal hyperplastic tissue counterparts for each case. All cultures were treated with two different amounts of Permixon® (44 and 88 μg/ml) for different time points (16, 24, 48 and 72 hours), depending on the cell type and the assay; the expression of inflammation-related genes, cell growth (proliferation/apoptosis ratio) and NF-κB activation has been analyzed in treated and untreated cells by means of semi-quantitative RNA-PCR, cell proliferation and immunofluorescence respectively. Results: We detected a significant reduction (p
KW - Apoptosis
KW - Caspase detection
KW - Cell cultures
KW - Inflammation
KW - Proliferation
KW - Prostate neoplasm
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U2 - 10.1186/1476-9255-10-11
DO - 10.1186/1476-9255-10-11
M3 - Article
VL - 10
JO - Journal of inflammation
JF - Journal of inflammation
SN - 1078-7852
IS - 1
M1 - 11
ER -