Effect of short course of 1,25-dihydroxyvitamin D3 on biochemical markers of bone remodelling in postmenopausal women

Paolo Sirtori, Corrado Sosio, Giuseppina Resmini, Alessandro Rubinacci

Research output: Contribution to journalArticlepeer-review

Abstract

This study was designed to test the hypothesis that a short treatment course of 1,25(OH)2D3 elicits a stimulation of osteoblast activity without any action on the osteoclast. To test this, oral daily doses of 0.5 μg or 1 μg of 1,25(OH)2D3 were administered for 7 days to two groups (n = 5 and n = 7, respectively) of postmenopausal women with low bone mineral density. Markers of osteoblast activity, i.e. osteocalcin (BGP), total alkaline phosphatase activity (ALP) and bone alkaline phosphatase activity (BALP), and markers of osteoclast activity, i.e. hydroxylysyl-pyridinoline (Pyr), lysyl-pyridoline (D-Pyr), and galactosyl-hydroxylysine (GHyl) were measured in plasma and in fasting urinary samples, respectively, at sequential times during and after 1,25(OH)2D3 administration. It resulted that short term 1 μg 1,25(OH)2D3 oral administration induced a significant (P <0.05) rise of BGP serum level without any associated increase of D-Pyr and GHyl, the latter also expressed as GHyl to GGHyl ratio. Urinary Pyr increased significantly after 1 μg daily doses of 1,25(OH)2D3. Thus, a short course of 1 μg daily doses of 1,25(OH)2D3 elicits a stimulation of osteoblast activity without any enhancement of D-Pyr, the most specific marker of osteoclast activity. The enhancement of Pyr after 1 μg daily doses of 1,25(OH)2D3 might be due to the activation of extraosseous metabolic pathways rather than to the activation of osteoclast.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalPharmacological Research
Volume33
Issue number6
DOIs
Publication statusPublished - Jun 1996

Keywords

  • 1,25(OH)D
  • Bone remodelling
  • Menopause
  • Osteopenia

ASJC Scopus subject areas

  • Pharmacology

Fingerprint

Dive into the research topics of 'Effect of short course of 1,25-dihydroxyvitamin D3 on biochemical markers of bone remodelling in postmenopausal women'. Together they form a unique fingerprint.

Cite this