Effect of sorbic acid administration on urinary trans,trans-muconic acid excretion in rats exposed to low levels of benzene

G. Marrubini, T. Coccini, L. Maestri, L. Manzo

Research output: Contribution to journalArticle

Abstract

Trans,trans-muconic acid (t,t-MA) is a biomarker of benzene exposure reflecting metabolic activation to trans,trans-muconaldehyde. t,t-MA background urinary levels are highly variable, thus limiting its use to exposure monitoring of levels over 1 ppm of benzene. Actually, sorbic acid (SA) is known to influence background excretion of t,t-MA in man, but only a few examples suggest that SA ingestion can enhance t,t-MA levels occurring together with benzene exposure. In this study, the effect of SA was investigated in benzene-exposed male Sprague-Dawley rats exposed to 1 ppm benzene for 6 h. Exposed animals had a 24-h urinary t,t-MA excretion higher than that observed in non-exposed animals (87±13 μg/kg vs 19±3 μg/kg body weight). The oral dose of 8 mg/kg body weight SA had no effect on urinary t,t-MA both in control and in benzene-exposed rats. Increases of t,t-MA levels in urine occurred at SA doses of 50-200 mg/kg body weight, and co-exposure to benzene and SA (50 and 100 mg/kg body weight) produced additive enhancement of t,t-MA excretion. These data demonstrate the dose-response relationship between SA administration and t,t-MA excretion. Our study showed that SA ingestion at doses equal to or greater than 50 mg/kg body weight significantly affects the t,t-MA urinary levels in rats exposed to 1 ppm of benzene for 6 h. These data support the conclusion that in man t,t-MA is not suitable for biomonitoring of low levels of benzene exposure.

Original languageEnglish
Pages (from-to)1799-1806
Number of pages8
JournalFood and Chemical Toxicology
Volume40
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

Sorbic Acid
sorbic acid
benzene
Benzene
Rats
excretion
acids
rats
Body Weight
body weight
Animals
muconic acid
dosage
Eating
ingestion
Environmental Monitoring
Biomarkers
dose response
Sprague Dawley Rats
mouth

Keywords

  • Benzene
  • Biological monitoring
  • Sorbic acid
  • Trans,trans-Muconic acid

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Effect of sorbic acid administration on urinary trans,trans-muconic acid excretion in rats exposed to low levels of benzene. / Marrubini, G.; Coccini, T.; Maestri, L.; Manzo, L.

In: Food and Chemical Toxicology, Vol. 40, No. 12, 01.12.2002, p. 1799-1806.

Research output: Contribution to journalArticle

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