Effect of stress on the paracellular barrier in the rat ileum

E. Mazzon, G. C. Sturniolo, D. Puzzolo, N. Frisina, Walter Fries

Research output: Contribution to journalArticlepeer-review


Background: Restraint stress induces permeability changes in the rat small intestine but little is known of the ultrastructural events leading to defects of the paracellular sealing or of the short term evolution of these alterations. Methods: In the present study, we performed transmission electron microscopy in the terminal ileum perfused with lanthanum after two hours of immobilisation stress and in non-stressed control rats. Moreover, immunohistochemistry of the tight junction (TJ) associated proteins, occludin and zonula occludens 1 (ZO-1), was carried out together with western blot analysis of the transmembrane protein occludin. TJ morphology was also assessed after a 22 hour recovery period. Results: Immobilisation stress induced a significant increase in epithelial permeability to the lanthanum tracer (p

Original languageEnglish
Pages (from-to)507-513
Number of pages7
Issue number4
Publication statusPublished - Oct 2002

ASJC Scopus subject areas

  • Gastroenterology


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