Effect of the different dimeric forms of the platelet-derived growth factor on cellular responses in mouse Swiss 3T3 fibroblasts

E. Sturani, R. Brambilla, L. Morello, M. G. Cattaneo, R. Zippel, L. Alberghina

Research output: Contribution to journalArticlepeer-review

Abstract

PDGF consists of two polypeptide chains, A and B, and all three possible dimers have been isolated from different sources. Human PDGF, essentially AB, porcine PDGF (BB) and recombinant PDGF-AA were tested on Swiss 3T3 fibroblasts for their ability to stimulate mitogenesis, phosphoinositide turnover and tyrosine phosphorylation of the PDGF receptor. When used in saturating amounts, the three isoforms were equally active in inducing mitogenesis. However, PDGF-AA was less active than AB and BB to induce the phosphorylation of the receptor and the turnover of phosphoinositides (30% and 50%, respectively). These findings suggest that, in Swiss 3T3 fibroblasts, PDGF receptors of the α-type are present in a slightly lower amount than β-type. In addition, the two types of receptor appear to have similar physiological functions.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalFEBS Letters
Volume255
Issue number1
DOIs
Publication statusPublished - Sep 11 1989

Keywords

  • (Mouse fibroblast)
  • Mitogenesis
  • Phosphoinositide
  • Platelet-derived growth factor isoform
  • Platelet-derived growth factor receptor
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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