Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility

Maria Carmen Puertas, Maria Jose Buzón, Anna Artese, Stefano Alcaro, Luis Menendez-Arias, Carlo Federico Perno, Bonaventura Clotet, Francesca Ceccherini-Silberstein, Javier Martinez-Picado

Research output: Contribution to journalArticlepeer-review

Abstract

A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.

Original languageEnglish
Pages (from-to)7434-7439
Number of pages6
JournalJournal of Virology
Volume83
Issue number15
DOIs
Publication statusPublished - Aug 2009

ASJC Scopus subject areas

  • Immunology
  • Virology

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