Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility

Maria Carmen Puertas; Maria Jose Buzón; Anna Artese; Stefano Alcaro; Luis Menendez-Arias; Carlo Federico Perno; Bonaventura Clotet; Francesca Ceccherini-Silberstein; Javier Martinez-Picado

doi:10.1128/JVI.00487-09

Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility

Maria Carmen Puertas, Maria Jose Buzón, Anna Artese, Stefano Alcaro, Luis Menendez-Arias, Carlo Federico Perno, Bonaventura Clotet, Francesca Ceccherini-Silberstein, Javier Martinez-Picado

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.

Original language	English
Pages (from-to)	7434-7439
Number of pages	6
Journal	Journal of Virology
Volume	83
Issue number	15
DOIs	https://doi.org/10.1128/JVI.00487-09
Publication status	Published - Aug 2009

ASJC Scopus subject areas

Immunology
Virology

Access to Document

10.1128/JVI.00487-09

Fingerprint Dive into the research topics of 'Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility'. Together they form a unique fingerprint.

Cite this

Puertas, M. C., Buzón, M. J., Artese, A., Alcaro, S., Menendez-Arias, L., Perno, C. F., Clotet, B., Ceccherini-Silberstein, F., & Martinez-Picado, J. (2009). Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility. Journal of Virology, 83(15), 7434-7439. https://doi.org/10.1128/JVI.00487-09

Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility. / Puertas, Maria Carmen; Buzón, Maria Jose; Artese, Anna; Alcaro, Stefano; Menendez-Arias, Luis; Perno, Carlo Federico; Clotet, Bonaventura; Ceccherini-Silberstein, Francesca; Martinez-Picado, Javier.

In: Journal of Virology, Vol. 83, No. 15, 08.2009, p. 7434-7439.

Research output: Contribution to journal › Article › peer-review

Puertas, MC, Buzón, MJ, Artese, A, Alcaro, S, Menendez-Arias, L, Perno, CF, Clotet, B, Ceccherini-Silberstein, F & Martinez-Picado, J 2009, 'Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility', Journal of Virology, vol. 83, no. 15, pp. 7434-7439. https://doi.org/10.1128/JVI.00487-09

Puertas, Maria Carmen ; Buzón, Maria Jose ; Artese, Anna ; Alcaro, Stefano ; Menendez-Arias, Luis ; Perno, Carlo Federico ; Clotet, Bonaventura ; Ceccherini-Silberstein, Francesca ; Martinez-Picado, Javier. / Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility. In: Journal of Virology. 2009 ; Vol. 83, No. 15. pp. 7434-7439.

@article{9b1b2033d55f42a3b310529944f2c03d,

title = "Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility",

abstract = "A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.",

author = "Puertas, {Maria Carmen} and Buz{\'o}n, {Maria Jose} and Anna Artese and Stefano Alcaro and Luis Menendez-Arias and Perno, {Carlo Federico} and Bonaventura Clotet and Francesca Ceccherini-Silberstein and Javier Martinez-Picado",

year = "2009",

month = aug,

doi = "10.1128/JVI.00487-09",

language = "English",

volume = "83",

pages = "7434--7439",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "15",

}

TY - JOUR

T1 - Effect of the human immunodeficiency virus type 1 reverse transcriptase polymorphism Leu-214 on replication capacity and drug susceptibility

AU - Puertas, Maria Carmen

AU - Buzón, Maria Jose

AU - Artese, Anna

AU - Alcaro, Stefano

AU - Menendez-Arias, Luis

AU - Perno, Carlo Federico

AU - Clotet, Bonaventura

AU - Ceccherini-Silberstein, Francesca

AU - Martinez-Picado, Javier

PY - 2009/8

Y1 - 2009/8

N2 - A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.

AB - A negative association between polymorphism Leu-214 and type-1 thymidine analogue mutations (TAM1) and a positive association with a clinically favorable virological response to thymidine analogue-based combination antiretroviral therapy have been described. In this study, the impact of Leu-214 on replication capacity and resistance to zidovudine (ZDV) of viruses containing TAM1 or TAM2 was determined. Leu-214 decreased the growth rate of viruses bearing Tyr-215, as well as their resistance to ZDV. This observation was confirmed by structural and molecular modeling data, suggesting a regulatory role for Leu-214 in the emergence and phenotypic resistance of TAM1.

UR - http://www.scopus.com/inward/record.url?scp=67650924884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650924884&partnerID=8YFLogxK

U2 - 10.1128/JVI.00487-09

DO - 10.1128/JVI.00487-09

M3 - Article

C2 - 19457988

AN - SCOPUS:67650924884

VL - 83

SP - 7434

EP - 7439

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 15

ER -