Introduction: ICSI is considered to be an appropriate treatment for infertile men with severe oligoasthenoteratozoospermia (OAT). Nevertheless, sperm morphological and functional alterations carried by these patients may impair ICSI success, such as high percentages of spermatozoa with DNA integrity anomalies. The efficiency of the available therapies for idiopathic OAT (iOAT) is still a matter for debate. It has been suggested that FSH therapy may be a specific pretreatment for infertile male partners of couples undergoing ICSI. Our study aimed to evaluate the effect of recombinant FSH (rFSH) on sperm DNA fragmentation in iOAT patients undergoing ICSI. Material and methods: 39 iOAT men volunteered for the study. Male patients were administered 150 IU of rFSH every other day for at least three months prior to ART. In order to determine the sperm DNA fragmentation index (DFI), TUNEL assay was performed before rFSH (baseline) and after the hormonal treatment (at the time of ICSI). Results and Conclusion: The analysis of data showed that after rFSH therapy a statistically significant reduction in the sperm DFI occurred (from 23.4±10.1 to 14.9±8.8). Three subsets of patients were identified on the basis of their DFI decrease. Our findings suggest that rFSH administration may improve sperm DNA integrity in iOAT men with increased DFI values.
|Translated title of the contribution||Effect of the therapy with recombinant FSH on the fragmentation of the spermatic DNA in patients with idiopathic oligoasthenoteratozoospermia undergoing ICSI|
|Number of pages||3|
|Journal||Giornale Italiano di Ostetricia e Ginecologia|
|Publication status||Published - 2009|
ASJC Scopus subject areas
- Obstetrics and Gynaecology