Effect of traumatic brain injury on cognitive function in mice lacking p55 and p75 tumor necrosis factor receptors

L. Longhi, F. Ortolano, E. R. Zanier, C. Perego, N. Stocchetti, M. G. De Simoni

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Background Tumor necrosis factor (TNF)-α has been suggested to play both a deleterious and beneficial role in neurobehavioral dysfunction and recovery following traumatic brain injury (TBI). The goal of this study was to evaluate the specific role of tumor necrosis factor (TNF) receptors p55 and p75 in mediating cognitive outcome following controlled cortical impact (CCI) brain injury by comparing post-traumatic cognitive function in mice with genetically engineered deletion of the gene for either p55 (-/-) or p75 (-/-) receptors. Method Male C57Bl/6 mice (WT, n=29), and mice genetically engineered to delete p55 TNF (p55 (-/-), n=8) or p75 TNF (p75 (-/-), n=23) receptors were used. They were anesthetized with intraperitoneal (i.p.) administration of sodium pentobarbital (65 mg/kg) and subjected to CCI brain injury of moderate severity. Sham-injured control mice were anesthetized and surgically prepared similarly but they received no impact. Assessment of mRNA expression of inflammatory, proapoptotic and antiapoptotic genes was done by real time-polymerase chain reaction (RT-PCR). Cognitive outcome was evaluated at 4 weeks postinjury using the Morris water maze (MWM). Findings mRNA expression of inflammatory, proapoptotic and antiapoptotic genes prior to TBI did not reveal any baseline difference between p55 and p75 (-/-) mice. WT mice showed greater baseline expression of inflammatory genes. The learning ability of p55 (-/-) brain-injured mice was significantly better than that observed in p75 (-/-) brain-injured mice (p

Original languageEnglish
Title of host publicationActa Neurochirurgica, Supplementum
Pages409-413
Number of pages5
Edition102
DOIs
Publication statusPublished - 2008

Publication series

NameActa Neurochirurgica, Supplementum
Number102
ISSN (Print)00651419

Keywords

  • Apoptosis
  • Inflammation
  • TNF-α
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Fingerprint

Dive into the research topics of 'Effect of traumatic brain injury on cognitive function in mice lacking p55 and p75 tumor necrosis factor receptors'. Together they form a unique fingerprint.

Cite this