Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells

A. Pessina, S. Eridani, P. Brambilla, G. Cattoretti, A. Marocchi, P. Mocarelli

Research output: Contribution to journalArticle

Abstract

The effect of normal mouse marrow preincubation with Ehrlich tumor fluid (EAF) and Ehrlich tumor cell-conditioned medium (ET-CM) was investigated both on the in vivo assay for pluripotential stem cells (CFU-S) and on the in vitro assay for granulocytic-macrophagic progenitors (CFU-C). A significant reduction of the number of CFU-S was found when bone marrow cells were incubated with EAF. The morphological analysis showed a relative increase in the granulocytic type colonies besides an absolute increase in undifferentiated ones and an absolute decrease in erythroid colonies. The in vitro data show a decrease in CFU-C production, by stimulation with standard Colony Stimulating Factor (CSF), when the marrow cells are preincubated with EAF and ET-CM. When standard CSF (obtained from L-cells) is used at the higher concentrations, the CFU-C in the ET-CM treated marrow increases to the control values EAF and ET-CM incubation also affects the CFU-C differentiation by increasing the number of pure granulocytic clones. The experimental data are discussed in order to explain an hypothesis about the activity of tumor-related factors.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalNeoplasma
Volume28
Issue number5
Publication statusPublished - 1981

Fingerprint

Bone Marrow
Conditioned Culture Medium
Colony-Stimulating Factors
Neoplasms
Colony-Forming Units Assay
Bone Marrow Cells
In Vitro Techniques
Clone Cells

ASJC Scopus subject areas

  • Cancer Research

Cite this

Pessina, A., Eridani, S., Brambilla, P., Cattoretti, G., Marocchi, A., & Mocarelli, P. (1981). Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells. Neoplasma, 28(5), 541-548.

Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells. / Pessina, A.; Eridani, S.; Brambilla, P.; Cattoretti, G.; Marocchi, A.; Mocarelli, P.

In: Neoplasma, Vol. 28, No. 5, 1981, p. 541-548.

Research output: Contribution to journalArticle

Pessina, A, Eridani, S, Brambilla, P, Cattoretti, G, Marocchi, A & Mocarelli, P 1981, 'Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells', Neoplasma, vol. 28, no. 5, pp. 541-548.
Pessina A, Eridani S, Brambilla P, Cattoretti G, Marocchi A, Mocarelli P. Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells. Neoplasma. 1981;28(5):541-548.
Pessina, A. ; Eridani, S. ; Brambilla, P. ; Cattoretti, G. ; Marocchi, A. ; Mocarelli, P. / Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells. In: Neoplasma. 1981 ; Vol. 28, No. 5. pp. 541-548.
@article{cdae3c86a6514bc98da25dc7891bc572,
title = "Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells",
abstract = "The effect of normal mouse marrow preincubation with Ehrlich tumor fluid (EAF) and Ehrlich tumor cell-conditioned medium (ET-CM) was investigated both on the in vivo assay for pluripotential stem cells (CFU-S) and on the in vitro assay for granulocytic-macrophagic progenitors (CFU-C). A significant reduction of the number of CFU-S was found when bone marrow cells were incubated with EAF. The morphological analysis showed a relative increase in the granulocytic type colonies besides an absolute increase in undifferentiated ones and an absolute decrease in erythroid colonies. The in vitro data show a decrease in CFU-C production, by stimulation with standard Colony Stimulating Factor (CSF), when the marrow cells are preincubated with EAF and ET-CM. When standard CSF (obtained from L-cells) is used at the higher concentrations, the CFU-C in the ET-CM treated marrow increases to the control values EAF and ET-CM incubation also affects the CFU-C differentiation by increasing the number of pure granulocytic clones. The experimental data are discussed in order to explain an hypothesis about the activity of tumor-related factors.",
author = "A. Pessina and S. Eridani and P. Brambilla and G. Cattoretti and A. Marocchi and P. Mocarelli",
year = "1981",
language = "English",
volume = "28",
pages = "541--548",
journal = "Neoplasma",
issn = "0028-2685",
publisher = "Vydavatel'stvo Slovenkej Akademie Vied/Veda Publishing House of the Slovak Academy of Sciences",
number = "5",

}

TY - JOUR

T1 - Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells

AU - Pessina, A.

AU - Eridani, S.

AU - Brambilla, P.

AU - Cattoretti, G.

AU - Marocchi, A.

AU - Mocarelli, P.

PY - 1981

Y1 - 1981

N2 - The effect of normal mouse marrow preincubation with Ehrlich tumor fluid (EAF) and Ehrlich tumor cell-conditioned medium (ET-CM) was investigated both on the in vivo assay for pluripotential stem cells (CFU-S) and on the in vitro assay for granulocytic-macrophagic progenitors (CFU-C). A significant reduction of the number of CFU-S was found when bone marrow cells were incubated with EAF. The morphological analysis showed a relative increase in the granulocytic type colonies besides an absolute increase in undifferentiated ones and an absolute decrease in erythroid colonies. The in vitro data show a decrease in CFU-C production, by stimulation with standard Colony Stimulating Factor (CSF), when the marrow cells are preincubated with EAF and ET-CM. When standard CSF (obtained from L-cells) is used at the higher concentrations, the CFU-C in the ET-CM treated marrow increases to the control values EAF and ET-CM incubation also affects the CFU-C differentiation by increasing the number of pure granulocytic clones. The experimental data are discussed in order to explain an hypothesis about the activity of tumor-related factors.

AB - The effect of normal mouse marrow preincubation with Ehrlich tumor fluid (EAF) and Ehrlich tumor cell-conditioned medium (ET-CM) was investigated both on the in vivo assay for pluripotential stem cells (CFU-S) and on the in vitro assay for granulocytic-macrophagic progenitors (CFU-C). A significant reduction of the number of CFU-S was found when bone marrow cells were incubated with EAF. The morphological analysis showed a relative increase in the granulocytic type colonies besides an absolute increase in undifferentiated ones and an absolute decrease in erythroid colonies. The in vitro data show a decrease in CFU-C production, by stimulation with standard Colony Stimulating Factor (CSF), when the marrow cells are preincubated with EAF and ET-CM. When standard CSF (obtained from L-cells) is used at the higher concentrations, the CFU-C in the ET-CM treated marrow increases to the control values EAF and ET-CM incubation also affects the CFU-C differentiation by increasing the number of pure granulocytic clones. The experimental data are discussed in order to explain an hypothesis about the activity of tumor-related factors.

UR - http://www.scopus.com/inward/record.url?scp=0019835326&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019835326&partnerID=8YFLogxK

M3 - Article

C2 - 6975896

AN - SCOPUS:0019835326

VL - 28

SP - 541

EP - 548

JO - Neoplasma

JF - Neoplasma

SN - 0028-2685

IS - 5

ER -