Effect of two different combinations of antiretrovirals (AZT+ddI and AZT+3TC) on cytokine production and apoptosis in asymptomatic HIV infection

Stefania Piconi, Daria Trabattoni, Maria Luisa Fusi, Francesco Milazzo, Lynn P. Dix, Giuliano Rizzardini, Fulvia Colombo, Dorothy Bray, Mario Clerici

Research output: Contribution to journalArticle

Abstract

Nineteen HIV-seropositive antiretroviral therapy-naive and asymptomatic individuals (200-500 CD4/μl) were enrolled in a prospective study aimed at analyzing the immunologic and virologic effects of two different combinations of nucleoside reverse transcriptase inhibitors (AZT+ddI and AZT+3TC), and randomly assigned to one of the treatment group. Immunologic (CD4 and CD8 counts, mitogen-stimulated cytokine production, unstimulated and mitogen-stimulated apoptosis) and virologic (HIV viral load) determinations were performed pre-therapy and 15, 30, 90, 200 and 360 days after initiation of therapy. Results showed that the two combinations had comparable effects on increasing CD4 counts and the CD4/CD8 ratio and in reducing HIV viral load. In contrast, AZT+3TC was more efficient in improving interleukin-2 (IL-2) and interferon gamma (IFNγ) production as well as the type 1/type 2 cytokine ratio and in down modulating the susceptibility of peripheral blood mononuclear cells to in vitro mitogen-stimulated apoptotic cell death. These data suggest that the combination of AZT+3TC has a stronger effect on potentially beneficial immune parameters (IL-2 production; reduction of apoptosis) than the one between AZT+ddI. The combination of AZT+3TC could be more advantageous in the therapy of HIV infection even when used in association with a protease inhibitor. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalAntiviral Research
Volume46
Issue number3
DOIs
Publication statusPublished - Jun 1 2000

Keywords

  • Antiviral therapy
  • Apoptosis
  • Cytokine
  • HIV infection
  • Immunology

ASJC Scopus subject areas

  • Virology
  • Pharmacology

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