Effect of two different glucose concentrations on insulin receptor mRNA levels in human hepatoma HepG2 cells

Paola Briata, Roberto Gherzi, Luciano Adezati, Renzo Cordera

Research output: Contribution to journalArticlepeer-review

Abstract

Glucose is known to affect mRNA levels of several genes. In order to investigate possible effects of glucose on insulin receptor mRNA levels, we cultured human hepatoma cells (HepG2) in two different culture media: DMEM containing 100 mg/dl glucose and DMEM containing 450 mg/dl glucose. Insulin receptor mRNA levels and insulin binding activity were reduced in HepG2 cultured at lower glucose concentrations. These data suggest that glucose affects insulin receptor gene expression.

Original languageEnglish
Pages (from-to)1415-1420
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number3
DOIs
Publication statusPublished - May 15 1989

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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