TY - JOUR
T1 - Effect of zinc supplementation on plasma IL-6 and MCP-1 production and NK cell function in healthy elderly
T2 - Interactive influence of +647 MT1a and -174 IL-6 polymorphic alleles
AU - Mariani, Erminia
AU - Neri, Simona
AU - Cattini, Luca
AU - Mocchegiani, Eugenio
AU - Malavolta, Marco
AU - Dedoussis, George V.
AU - Kanoni, Stavroula
AU - Rink, Lothar
AU - Jajte, Jolanta
AU - Facchini, Andrea
PY - 2008/5
Y1 - 2008/5
N2 - Pro-inflammatory cytokine response and NK activity are controlled by the availability of zinc ion, whose intra-cellular transport is regulated by metallothioneins. In order to closely examine the importance of circulating zinc in the modulation of immune response during ageing, in the balance of Th2/Th1 equilibrium and finally in the reversibility of systemic low grade inflammation, we evaluated the changes occurring in plasma IL-6 and MCP-1 concentrations and NK lytic activity in a healthy low grade inflamed elderly population, following zinc-aspartate supplementation. In addition, we aimed to highlight the potential interaction among circulating zinc increments, changes in immunological parameters and +647 MT1a and -174 IL-6 polymorphic alleles. Thirty-nine healthy individuals (60-83 years) from the ZINCAGE cohort (previously typed for +647 MT1a and -174 IL-6 polymorphisms) were supplied with zinc-aspartate. Blood samples collected before and after supplementation underwent basal laboratory determinations (circulating zinc, albumin and C-reactive protein) and immunological studies (plasma IL-6 and MCP-1 and NK lytic activity). Zinc supplementation in subjects with low or borderline-normal circulating zinc increased the concentration of this ion and modulated plasmatic IL-6 and MCP-1 as well as NK lytic activity. An interactive effect of polymorphic alleles of MT1a and IL-6 genes on zinc, IL-6, MCP-1 and NK activity was evidenced following supplementation, indicating the genetic background as one of the determinants for identifying groups of subjects that can take advantage of therapeutic intervention.
AB - Pro-inflammatory cytokine response and NK activity are controlled by the availability of zinc ion, whose intra-cellular transport is regulated by metallothioneins. In order to closely examine the importance of circulating zinc in the modulation of immune response during ageing, in the balance of Th2/Th1 equilibrium and finally in the reversibility of systemic low grade inflammation, we evaluated the changes occurring in plasma IL-6 and MCP-1 concentrations and NK lytic activity in a healthy low grade inflamed elderly population, following zinc-aspartate supplementation. In addition, we aimed to highlight the potential interaction among circulating zinc increments, changes in immunological parameters and +647 MT1a and -174 IL-6 polymorphic alleles. Thirty-nine healthy individuals (60-83 years) from the ZINCAGE cohort (previously typed for +647 MT1a and -174 IL-6 polymorphisms) were supplied with zinc-aspartate. Blood samples collected before and after supplementation underwent basal laboratory determinations (circulating zinc, albumin and C-reactive protein) and immunological studies (plasma IL-6 and MCP-1 and NK lytic activity). Zinc supplementation in subjects with low or borderline-normal circulating zinc increased the concentration of this ion and modulated plasmatic IL-6 and MCP-1 as well as NK lytic activity. An interactive effect of polymorphic alleles of MT1a and IL-6 genes on zinc, IL-6, MCP-1 and NK activity was evidenced following supplementation, indicating the genetic background as one of the determinants for identifying groups of subjects that can take advantage of therapeutic intervention.
KW - +647 MT1a polymorphism
KW - -174 IL-6 polymorphism
KW - Elderly
KW - IL-6
KW - MCP-1
KW - NK lytic activity
KW - Zinc
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UR - http://www.scopus.com/inward/citedby.url?scp=42149193103&partnerID=8YFLogxK
U2 - 10.1016/j.exger.2007.12.003
DO - 10.1016/j.exger.2007.12.003
M3 - Article
C2 - 18215484
AN - SCOPUS:42149193103
VL - 43
SP - 462
EP - 471
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
IS - 5
ER -