Effectiveness of immunohistochemical techniques in the detection of lymph node micrometastases of cervical squamous cell carcinoma

G. Lapertosa, P. Baracchini, F. Cabiddu, E. Fulcheri, F. Pedulla, N. Ragni

Research output: Contribution to journalArticle

Abstract

Out of a total of 1050 lymph nodes from patients with clinical squamous carcinoma, 58 lymph node metastases related to 18 patients were detected with H&E staining, while 61 lymph node metastases related to 19 patients were observed by immunohistochemical methods. However, by re-staining the original H&E sections with an anti-keratin antibody, the same 58 lymph node metastases were seen. Therefore, the three metastases emerged only in deeper sections which had been used for the immunohistochemical examination. It is suggested that the detection of micrometastases in lymph nodes may be improved by examining several sections at different levels rather than by immunohistochemical methods, even though these facilitate the observation of small groups of neoplastic cells.

Original languageEnglish
Pages (from-to)33-46
Number of pages14
JournalInternational Journal of Gynecological Cancer
Volume1
Issue number1
Publication statusPublished - 1991

Fingerprint

Neoplasm Micrometastasis
Squamous Cell Carcinoma
Lymph Nodes
Neoplasm Metastasis
Staining and Labeling
Keratins
Anti-Idiotypic Antibodies
Observation

Keywords

  • Immunohistochemistry
  • Keratin antibodies
  • Lymph node
  • Micrometastases
  • Squamous cell carcinoma
  • Uterine cervix

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

Cite this

Effectiveness of immunohistochemical techniques in the detection of lymph node micrometastases of cervical squamous cell carcinoma. / Lapertosa, G.; Baracchini, P.; Cabiddu, F.; Fulcheri, E.; Pedulla, F.; Ragni, N.

In: International Journal of Gynecological Cancer, Vol. 1, No. 1, 1991, p. 33-46.

Research output: Contribution to journalArticle

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AU - Ragni, N.

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