Abstract
Out of a total of 1050 lymph nodes from patients with clinical squamous carcinoma, 58 lymph node metastases related to 18 patients were detected with H&E staining, while 61 lymph node metastases related to 19 patients were observed by immunohistochemical methods. However, by re-staining the original H&E sections with an anti-keratin antibody, the same 58 lymph node metastases were seen. Therefore, the three metastases emerged only in deeper sections which had been used for the immunohistochemical examination. It is suggested that the detection of micrometastases in lymph nodes may be improved by examining several sections at different levels rather than by immunohistochemical methods, even though these facilitate the observation of small groups of neoplastic cells.
| Original language | English |
|---|---|
| Pages (from-to) | 33-46 |
| Number of pages | 14 |
| Journal | International Journal of Gynecological Cancer |
| Volume | 1 |
| Issue number | 1 |
| Publication status | Published - 1991 |
Fingerprint
Keywords
- Immunohistochemistry
- Keratin antibodies
- Lymph node
- Micrometastases
- Squamous cell carcinoma
- Uterine cervix
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Oncology
Cite this
Effectiveness of immunohistochemical techniques in the detection of lymph node micrometastases of cervical squamous cell carcinoma. / Lapertosa, G.; Baracchini, P.; Cabiddu, F.; Fulcheri, E.; Pedulla, F.; Ragni, N.
In: International Journal of Gynecological Cancer, Vol. 1, No. 1, 1991, p. 33-46.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effectiveness of immunohistochemical techniques in the detection of lymph node micrometastases of cervical squamous cell carcinoma
AU - Lapertosa, G.
AU - Baracchini, P.
AU - Cabiddu, F.
AU - Fulcheri, E.
AU - Pedulla, F.
AU - Ragni, N.
PY - 1991
Y1 - 1991
N2 - Out of a total of 1050 lymph nodes from patients with clinical squamous carcinoma, 58 lymph node metastases related to 18 patients were detected with H&E staining, while 61 lymph node metastases related to 19 patients were observed by immunohistochemical methods. However, by re-staining the original H&E sections with an anti-keratin antibody, the same 58 lymph node metastases were seen. Therefore, the three metastases emerged only in deeper sections which had been used for the immunohistochemical examination. It is suggested that the detection of micrometastases in lymph nodes may be improved by examining several sections at different levels rather than by immunohistochemical methods, even though these facilitate the observation of small groups of neoplastic cells.
AB - Out of a total of 1050 lymph nodes from patients with clinical squamous carcinoma, 58 lymph node metastases related to 18 patients were detected with H&E staining, while 61 lymph node metastases related to 19 patients were observed by immunohistochemical methods. However, by re-staining the original H&E sections with an anti-keratin antibody, the same 58 lymph node metastases were seen. Therefore, the three metastases emerged only in deeper sections which had been used for the immunohistochemical examination. It is suggested that the detection of micrometastases in lymph nodes may be improved by examining several sections at different levels rather than by immunohistochemical methods, even though these facilitate the observation of small groups of neoplastic cells.
KW - Immunohistochemistry
KW - Keratin antibodies
KW - Lymph node
KW - Micrometastases
KW - Squamous cell carcinoma
KW - Uterine cervix
UR - http://www.scopus.com/inward/record.url?scp=84930307879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930307879&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84930307879
VL - 1
SP - 33
EP - 46
JO - International Journal of Gynecological Cancer
JF - International Journal of Gynecological Cancer
SN - 1048-891X
IS - 1
ER -