Effectiveness of leukocyte filters in reducing tumor cell contamination after intraoperative blood salvage in lung cancer patients

P. Perseghin, M. Viganò, G. Rocco, C. Della Pona, A. Buscemi, A. Rizzi

Research output: Contribution to journalArticle

Abstract

Objectives: In an effort to reduce allogeneic blood transfusions in patients undergoing elective surgery for lung cancer, we investigated the effectiveness of a method of processing shed blood with an automated device for intra operative blood salvage (IOBS) and filtration with a 3rd-generation polyester filter to remove tumor cells. Methods: Sixteen patients were operated on for different types of lung cancer. We searched for malignant cells in pre- and postprocessed shed blood employing density gradient centrifugation, staining of cytospins with hematoxylin-eosin, and antibodies to human cytokeratins. Results: In 9 out of 16 cases (56%), neoplastic cells were detected in prefiltration samples, but none were found in postfiltration cytospins. Conclusion: IOBS combined with appropriate filtration could be a very useful and safe tool in reducing allogeneic blood transfusion in cancer patients.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalVox Sanguinis
Volume72
Issue number4
Publication statusPublished - Jun 1997

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Operative Blood Salvage
Lung Neoplasms
Leukocytes
Blood Transfusion
Neoplasms
Polyesters
Density Gradient Centrifugation
Hematoxylin
Eosine Yellowish-(YS)
Keratins
Staining and Labeling
Equipment and Supplies
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Effectiveness of leukocyte filters in reducing tumor cell contamination after intraoperative blood salvage in lung cancer patients. / Perseghin, P.; Viganò, M.; Rocco, G.; Della Pona, C.; Buscemi, A.; Rizzi, A.

In: Vox Sanguinis, Vol. 72, No. 4, 06.1997, p. 221-224.

Research output: Contribution to journalArticle

Perseghin, P, Viganò, M, Rocco, G, Della Pona, C, Buscemi, A & Rizzi, A 1997, 'Effectiveness of leukocyte filters in reducing tumor cell contamination after intraoperative blood salvage in lung cancer patients', Vox Sanguinis, vol. 72, no. 4, pp. 221-224.
Perseghin, P. ; Viganò, M. ; Rocco, G. ; Della Pona, C. ; Buscemi, A. ; Rizzi, A. / Effectiveness of leukocyte filters in reducing tumor cell contamination after intraoperative blood salvage in lung cancer patients. In: Vox Sanguinis. 1997 ; Vol. 72, No. 4. pp. 221-224.
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