Abstract
Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.
| Original language | English |
|---|---|
| Pages (from-to) | 300-303 |
| Number of pages | 4 |
| Journal | Nature Biotechnology |
| Volume | 18 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- Anti-gene
- C-myc
- Lymphomas
- Nuclear localization signal (NLS) peptide
- Peptide nucleic acid (PNA)
ASJC Scopus subject areas
- Microbiology
Cite this
Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal. / Cutrona, Giovanna; Carpaneto, Elisabetta M.; Ulivi, Massimo; Roncella, Silvio; Landt, Olfert; Ferrarini, Manlio; Boffa, Lidia C.
In: Nature Biotechnology, Vol. 18, No. 3, 2000, p. 300-303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal
AU - Cutrona, Giovanna
AU - Carpaneto, Elisabetta M.
AU - Ulivi, Massimo
AU - Roncella, Silvio
AU - Landt, Olfert
AU - Ferrarini, Manlio
AU - Boffa, Lidia C.
PY - 2000
Y1 - 2000
N2 - Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.
AB - Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.
KW - Anti-gene
KW - C-myc
KW - Lymphomas
KW - Nuclear localization signal (NLS) peptide
KW - Peptide nucleic acid (PNA)
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UR - http://www.scopus.com/inward/citedby.url?scp=0034061532&partnerID=8YFLogxK
U2 - 10.1038/73745
DO - 10.1038/73745
M3 - Article
C2 - 10700145
AN - SCOPUS:0034061532
VL - 18
SP - 300
EP - 303
JO - Biotechnology
JF - Biotechnology
SN - 1087-0156
IS - 3
ER -