Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal

Giovanna Cutrona, Elisabetta M. Carpaneto, Massimo Ulivi, Silvio Roncella, Olfert Landt, Manlio Ferrarini, Lidia C. Boffa

Research output: Contribution to journalArticle

Abstract

Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.

Original languageEnglish
Pages (from-to)300-303
Number of pages4
JournalNature Biotechnology
Volume18
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Peptide Nucleic Acids
Nuclear Localization Signals
myc Genes
Genes
Burkitt Lymphoma
Cells
Sugar Phosphates
Polynucleotides
Nucleic acids
Nylons
Cell death
Protein Sorting Signals
Cell Nucleus
Polyamides
Oncogenes
Cell culture
Nucleic Acids
Exons
Cell Cycle
Down-Regulation

Keywords

  • Anti-gene
  • C-myc
  • Lymphomas
  • Nuclear localization signal (NLS) peptide
  • Peptide nucleic acid (PNA)

ASJC Scopus subject areas

  • Microbiology

Cite this

Cutrona, G., Carpaneto, E. M., Ulivi, M., Roncella, S., Landt, O., Ferrarini, M., & Boffa, L. C. (2000). Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal. Nature Biotechnology, 18(3), 300-303. https://doi.org/10.1038/73745

Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal. / Cutrona, Giovanna; Carpaneto, Elisabetta M.; Ulivi, Massimo; Roncella, Silvio; Landt, Olfert; Ferrarini, Manlio; Boffa, Lidia C.

In: Nature Biotechnology, Vol. 18, No. 3, 2000, p. 300-303.

Research output: Contribution to journalArticle

Cutrona, G, Carpaneto, EM, Ulivi, M, Roncella, S, Landt, O, Ferrarini, M & Boffa, LC 2000, 'Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal', Nature Biotechnology, vol. 18, no. 3, pp. 300-303. https://doi.org/10.1038/73745
Cutrona, Giovanna ; Carpaneto, Elisabetta M. ; Ulivi, Massimo ; Roncella, Silvio ; Landt, Olfert ; Ferrarini, Manlio ; Boffa, Lidia C. / Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal. In: Nature Biotechnology. 2000 ; Vol. 18, No. 3. pp. 300-303.
@article{2398073ad9f548c4a51b81b559966dba,
title = "Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal",
abstract = "Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.",
keywords = "Anti-gene, C-myc, Lymphomas, Nuclear localization signal (NLS) peptide, Peptide nucleic acid (PNA)",
author = "Giovanna Cutrona and Carpaneto, {Elisabetta M.} and Massimo Ulivi and Silvio Roncella and Olfert Landt and Manlio Ferrarini and Boffa, {Lidia C.}",
year = "2000",
doi = "10.1038/73745",
language = "English",
volume = "18",
pages = "300--303",
journal = "Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal

AU - Cutrona, Giovanna

AU - Carpaneto, Elisabetta M.

AU - Ulivi, Massimo

AU - Roncella, Silvio

AU - Landt, Olfert

AU - Ferrarini, Manlio

AU - Boffa, Lidia C.

PY - 2000

Y1 - 2000

N2 - Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.

AB - Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c- myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti- gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.

KW - Anti-gene

KW - C-myc

KW - Lymphomas

KW - Nuclear localization signal (NLS) peptide

KW - Peptide nucleic acid (PNA)

UR - http://www.scopus.com/inward/record.url?scp=0034061532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034061532&partnerID=8YFLogxK

U2 - 10.1038/73745

DO - 10.1038/73745

M3 - Article

C2 - 10700145

AN - SCOPUS:0034061532

VL - 18

SP - 300

EP - 303

JO - Biotechnology

JF - Biotechnology

SN - 1087-0156

IS - 3

ER -