Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach

Anna Chiara Nittoli, Susan Costantini, Angela Sorice, Francesca Capone, Roberto Ciarcia, Stefania Marzocco, Alfredo Budillon, Lorella Severino

Research output: Contribution to journalArticle

Abstract

Introduction: Zearalenone (ZEN) is one of the most widely distributed toxins that contaminates many crops and foods. Its major metabolites are α-Zearalenol (α-zol) and β-Zearalenol. Previous studies showed that ZEN and α-zol have estrogenic properties and are able to induce growth promoting effect in breast tissues. Objectivies: Considering that tumorigenesis is dependent on the reprogramming of cellular metabolism and that the evaluation of the cellular metabolome is useful to understand the metabolic changes that can occur during the cancer development and progression or after treatments, aim of our work is to study, for the first time, the effects of α-zol on the metabolomic profile of an estrogen positive breast cancer cell line, MCF-7, and of an estrogen negative breast cancer cell lines MDA-MB231. Methods: Firstly, we tested the effects of α-zol on the cell viability after 24, 48 and 72 h of treatments with 10−10, 10−8 and 10−6 M concentrations on breast cancer MCF-7 and MDA-MB231 cell lines in comparison to human non-cancerous breast MCF10A cell line. Then, we evaluated cell cycle progression, levels of reactive oxygen species (ROS) and the metabolomic profiling by 1H-NMR approach on MCF-7 and MDA-MB231 before and after 72 h treatments. Principal component analysis was used to compare the obtained spectra. Results: α-zol is resulted able to induce: (i) an increase of the cell viability on MCF-7 cells mainly after 72 h treatment, (ii) a slight decrease of the cell viability on MDA-MB231 cells, and (iii) an increase of cells in S phase of the cell cycle and of ROS only in MCF-7 cells. Moreover, the evaluation of metabolomics profile evidenced that after treatment with α-zol the levels of some metabolites increased in MCF-7 cells whereas decreased slightly in MDA-MB231 cells. Conclusions: Our results showed that α-zol was able to increase the protein biosynthesis as well as the lipid metabolism in MCF-7 cells, and, hence, to induce an estrogen positive breast cancer progression.

Original languageEnglish
Article number33
JournalMetabolomics
Volume14
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Metabolome
MCF-7 Cells
Metabolomics
Cells
Nuclear magnetic resonance
Breast Neoplasms
Zearalenone
Cell Line
Cell Survival
Estrogens
Reactive Oxygen Species
Cell Cycle
Breast
Protein Biosynthesis
Principal Component Analysis
S Phase
Lipid Metabolism
Metabolites
Carcinogenesis
Food

Keywords

  • Alpha-zol
  • Breast cancer
  • Metabolome
  • NMR

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry

Cite this

Nittoli, A. C., Costantini, S., Sorice, A., Capone, F., Ciarcia, R., Marzocco, S., ... Severino, L. (2018). Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. Metabolomics, 14(3), [33]. https://doi.org/10.1007/s11306-018-1330-3

Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. / Nittoli, Anna Chiara; Costantini, Susan; Sorice, Angela; Capone, Francesca; Ciarcia, Roberto; Marzocco, Stefania; Budillon, Alfredo; Severino, Lorella.

In: Metabolomics, Vol. 14, No. 3, 33, 01.03.2018.

Research output: Contribution to journalArticle

Nittoli, AC, Costantini, S, Sorice, A, Capone, F, Ciarcia, R, Marzocco, S, Budillon, A & Severino, L 2018, 'Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach', Metabolomics, vol. 14, no. 3, 33. https://doi.org/10.1007/s11306-018-1330-3
Nittoli AC, Costantini S, Sorice A, Capone F, Ciarcia R, Marzocco S et al. Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. Metabolomics. 2018 Mar 1;14(3). 33. https://doi.org/10.1007/s11306-018-1330-3
Nittoli, Anna Chiara ; Costantini, Susan ; Sorice, Angela ; Capone, Francesca ; Ciarcia, Roberto ; Marzocco, Stefania ; Budillon, Alfredo ; Severino, Lorella. / Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. In: Metabolomics. 2018 ; Vol. 14, No. 3.
@article{a320b9e0842544d092158314fa3949af,
title = "Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach",
abstract = "Introduction: Zearalenone (ZEN) is one of the most widely distributed toxins that contaminates many crops and foods. Its major metabolites are α-Zearalenol (α-zol) and β-Zearalenol. Previous studies showed that ZEN and α-zol have estrogenic properties and are able to induce growth promoting effect in breast tissues. Objectivies: Considering that tumorigenesis is dependent on the reprogramming of cellular metabolism and that the evaluation of the cellular metabolome is useful to understand the metabolic changes that can occur during the cancer development and progression or after treatments, aim of our work is to study, for the first time, the effects of α-zol on the metabolomic profile of an estrogen positive breast cancer cell line, MCF-7, and of an estrogen negative breast cancer cell lines MDA-MB231. Methods: Firstly, we tested the effects of α-zol on the cell viability after 24, 48 and 72 h of treatments with 10−10, 10−8 and 10−6 M concentrations on breast cancer MCF-7 and MDA-MB231 cell lines in comparison to human non-cancerous breast MCF10A cell line. Then, we evaluated cell cycle progression, levels of reactive oxygen species (ROS) and the metabolomic profiling by 1H-NMR approach on MCF-7 and MDA-MB231 before and after 72 h treatments. Principal component analysis was used to compare the obtained spectra. Results: α-zol is resulted able to induce: (i) an increase of the cell viability on MCF-7 cells mainly after 72 h treatment, (ii) a slight decrease of the cell viability on MDA-MB231 cells, and (iii) an increase of cells in S phase of the cell cycle and of ROS only in MCF-7 cells. Moreover, the evaluation of metabolomics profile evidenced that after treatment with α-zol the levels of some metabolites increased in MCF-7 cells whereas decreased slightly in MDA-MB231 cells. Conclusions: Our results showed that α-zol was able to increase the protein biosynthesis as well as the lipid metabolism in MCF-7 cells, and, hence, to induce an estrogen positive breast cancer progression.",
keywords = "Alpha-zol, Breast cancer, Metabolome, NMR",
author = "Nittoli, {Anna Chiara} and Susan Costantini and Angela Sorice and Francesca Capone and Roberto Ciarcia and Stefania Marzocco and Alfredo Budillon and Lorella Severino",
year = "2018",
month = "3",
day = "1",
doi = "10.1007/s11306-018-1330-3",
language = "English",
volume = "14",
journal = "Metabolomics",
issn = "1573-3882",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach

AU - Nittoli, Anna Chiara

AU - Costantini, Susan

AU - Sorice, Angela

AU - Capone, Francesca

AU - Ciarcia, Roberto

AU - Marzocco, Stefania

AU - Budillon, Alfredo

AU - Severino, Lorella

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Introduction: Zearalenone (ZEN) is one of the most widely distributed toxins that contaminates many crops and foods. Its major metabolites are α-Zearalenol (α-zol) and β-Zearalenol. Previous studies showed that ZEN and α-zol have estrogenic properties and are able to induce growth promoting effect in breast tissues. Objectivies: Considering that tumorigenesis is dependent on the reprogramming of cellular metabolism and that the evaluation of the cellular metabolome is useful to understand the metabolic changes that can occur during the cancer development and progression or after treatments, aim of our work is to study, for the first time, the effects of α-zol on the metabolomic profile of an estrogen positive breast cancer cell line, MCF-7, and of an estrogen negative breast cancer cell lines MDA-MB231. Methods: Firstly, we tested the effects of α-zol on the cell viability after 24, 48 and 72 h of treatments with 10−10, 10−8 and 10−6 M concentrations on breast cancer MCF-7 and MDA-MB231 cell lines in comparison to human non-cancerous breast MCF10A cell line. Then, we evaluated cell cycle progression, levels of reactive oxygen species (ROS) and the metabolomic profiling by 1H-NMR approach on MCF-7 and MDA-MB231 before and after 72 h treatments. Principal component analysis was used to compare the obtained spectra. Results: α-zol is resulted able to induce: (i) an increase of the cell viability on MCF-7 cells mainly after 72 h treatment, (ii) a slight decrease of the cell viability on MDA-MB231 cells, and (iii) an increase of cells in S phase of the cell cycle and of ROS only in MCF-7 cells. Moreover, the evaluation of metabolomics profile evidenced that after treatment with α-zol the levels of some metabolites increased in MCF-7 cells whereas decreased slightly in MDA-MB231 cells. Conclusions: Our results showed that α-zol was able to increase the protein biosynthesis as well as the lipid metabolism in MCF-7 cells, and, hence, to induce an estrogen positive breast cancer progression.

AB - Introduction: Zearalenone (ZEN) is one of the most widely distributed toxins that contaminates many crops and foods. Its major metabolites are α-Zearalenol (α-zol) and β-Zearalenol. Previous studies showed that ZEN and α-zol have estrogenic properties and are able to induce growth promoting effect in breast tissues. Objectivies: Considering that tumorigenesis is dependent on the reprogramming of cellular metabolism and that the evaluation of the cellular metabolome is useful to understand the metabolic changes that can occur during the cancer development and progression or after treatments, aim of our work is to study, for the first time, the effects of α-zol on the metabolomic profile of an estrogen positive breast cancer cell line, MCF-7, and of an estrogen negative breast cancer cell lines MDA-MB231. Methods: Firstly, we tested the effects of α-zol on the cell viability after 24, 48 and 72 h of treatments with 10−10, 10−8 and 10−6 M concentrations on breast cancer MCF-7 and MDA-MB231 cell lines in comparison to human non-cancerous breast MCF10A cell line. Then, we evaluated cell cycle progression, levels of reactive oxygen species (ROS) and the metabolomic profiling by 1H-NMR approach on MCF-7 and MDA-MB231 before and after 72 h treatments. Principal component analysis was used to compare the obtained spectra. Results: α-zol is resulted able to induce: (i) an increase of the cell viability on MCF-7 cells mainly after 72 h treatment, (ii) a slight decrease of the cell viability on MDA-MB231 cells, and (iii) an increase of cells in S phase of the cell cycle and of ROS only in MCF-7 cells. Moreover, the evaluation of metabolomics profile evidenced that after treatment with α-zol the levels of some metabolites increased in MCF-7 cells whereas decreased slightly in MDA-MB231 cells. Conclusions: Our results showed that α-zol was able to increase the protein biosynthesis as well as the lipid metabolism in MCF-7 cells, and, hence, to induce an estrogen positive breast cancer progression.

KW - Alpha-zol

KW - Breast cancer

KW - Metabolome

KW - NMR

UR - http://www.scopus.com/inward/record.url?scp=85042139819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042139819&partnerID=8YFLogxK

U2 - 10.1007/s11306-018-1330-3

DO - 10.1007/s11306-018-1330-3

M3 - Article

AN - SCOPUS:85042139819

VL - 14

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 3

M1 - 33

ER -