Effects of δ-tocotrienol on ochratoxin A-induced nephrotoxicity in rats

Sara Damiano, Luigi Navas, Patrizia Lombari, Serena Montagnaro, Iris M. Forte, Antonio Giordano, Salvatore Florio, Roberto Ciarcia

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ochratoxin A (OTA), is a natural contaminant of the food chain worldwide involved in the development of different type of cancers in animals and humans. Several studies suggested that oxidative damage might contribute to increase the cytotoxicity and carcinogenicity capabilities of OTA. The aim of this study was to evaluate the possible protective effect of δ-tocotrienol (Delta), a natural form of vitamin E, against OTA-induced nephrotoxicity. Male Sprague-Dawley rats were treated with OTA and/or Delta by gavage for 14 days. Our results shown that OTA treatment induced the increase of reactive oxigen species production correlated to a strong reduction of Glomerular Filtration Rate (GFR) and absoluted fluid reabsorption (Jv) with conseguent significant increase in blood pressure. Consistent, we noted in the kidney of rats treated with OTA, an increase in malondialdheyde and dihydroethidium production and a reduction of the activity of the catalase, superoxide dismutase, and glutathione peroxidase. Conversly, in the rat group subjected to the concomitant treatment OTA plus Delta, we observed the restored effect, compared the OTA treatment group, on blood pressure, GFR, Jv, and all activities of renal antioxidant enzymes. Finally, as far as concern the tissue damage induced by OTA and measured evaluating fibronectin protein levels, we observed that in OTA plus Delta group this effect is not restored. Our findings releval that a mechanism underlying the renal toxicity induced by OTA is the oxidative stress and provide a new rationale to use a Delta in order to protect, at least in part, against OTA-induced nephrotoxicity.

Original languageEnglish
JournalJournal of Cellular Physiology
DOIs
Publication statusPublished - 2018

Fingerprint

Tocotrienols
Rats
Blood pressure
Glomerular Filtration Rate
Kidney
ochratoxin A
Blood Pressure
Oxidative stress
Food Chain
Cytotoxicity
Glutathione Peroxidase
Vitamin E
Fibronectins
Catalase
Superoxide Dismutase
Sprague Dawley Rats
Toxicity

Keywords

  • Nephrotoxicity
  • Ochratoxin A
  • Reactive oxigen species
  • δ-tocotrienol

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Damiano, S., Navas, L., Lombari, P., Montagnaro, S., Forte, I. M., Giordano, A., ... Ciarcia, R. (2018). Effects of δ-tocotrienol on ochratoxin A-induced nephrotoxicity in rats. Journal of Cellular Physiology. https://doi.org/10.1002/jcp.26753

Effects of δ-tocotrienol on ochratoxin A-induced nephrotoxicity in rats. / Damiano, Sara; Navas, Luigi; Lombari, Patrizia; Montagnaro, Serena; Forte, Iris M.; Giordano, Antonio; Florio, Salvatore; Ciarcia, Roberto.

In: Journal of Cellular Physiology, 2018.

Research output: Contribution to journalArticle

Damiano, S, Navas, L, Lombari, P, Montagnaro, S, Forte, IM, Giordano, A, Florio, S & Ciarcia, R 2018, 'Effects of δ-tocotrienol on ochratoxin A-induced nephrotoxicity in rats', Journal of Cellular Physiology. https://doi.org/10.1002/jcp.26753
Damiano, Sara ; Navas, Luigi ; Lombari, Patrizia ; Montagnaro, Serena ; Forte, Iris M. ; Giordano, Antonio ; Florio, Salvatore ; Ciarcia, Roberto. / Effects of δ-tocotrienol on ochratoxin A-induced nephrotoxicity in rats. In: Journal of Cellular Physiology. 2018.
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