Effects of 7β-estradiol on cytokine-induced endothelial cell adhesion molecule expression

T. Caulin-Glaser, C. A. Watson, R. Pardi, J. R. Bender

Research output: Contribution to journalArticlepeer-review

Abstract

One of the earliest events in atherosclerosis is interaction of circulating mononuclear leukocytes and the endothelium. Endothelial cell (EC) activation by cytokines results in expression of adhesion molecules and production of chemotactic factors, augmenting leukocyte adhesion and recruitment, respectively. The incidence of atherosclerosis in premenopausal women is significantly less than that observed in age-matched males with similar risk profiles. Because estrogen has gene regulatory effects, we investigated whether 17β-estradiol (E2) can inhibit cytokine-mediated EC adhesion molecule transcriptional activation. Cultured human umbilical vein EC (estrogen receptor-positive) were propagated in gonadal hormone-free medium and were E2-pretreated for 48 hr before IL-1 activation. Detected by FACS® analysis, E2 strongly (60-80%) inhibited IL-1-mediated membrane E- selectin and vascular cell adhesion molecule-1 induction, and intercellular adhesion molecule-1 hyperinduction. 17α-estradiol (an inactive E2 stereoisomer) had no effect. This inhibition correlated with similar reductions in steady state-induced E-selectin mRNA levels, and was abrogated by the E2 antagonist ICI 164,384, demonstrating a specific, estrogen receptor-mediated effect. Nuclear runoffs confirmed suppression at the transcriptional level. The implications of these results for the cardiovascular protective role of estrogen are discussed.

Original languageEnglish
Pages (from-to)36-42
Number of pages7
JournalJournal of Clinical Investigation
Volume98
Issue number1
Publication statusPublished - Jul 1 1996

Keywords

  • atherosclerosis
  • estrogen receptor
  • gene regulation
  • leukocyte
  • vascular endothelium

ASJC Scopus subject areas

  • Medicine(all)

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