Effects of a 50 Hz Sinusoidal Magnetic Field on Cell Adhesion Molecule Expression in Two Human Osteosarcoma Cell Lines (MG-63 and Saos-2)

Maria Teresa Santini, Gabriella Rainaldi, Antonella Ferrante, Pietro Luigi Indovina, Paolo Vecchia, Gianfranco Donelli

Research output: Contribution to journalArticle

Abstract

The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor for collagen, and VLA-5, the receptor for fibronectin, as well as CD44, were examined in both cell lines after these had been exposed for 7 and 14 days to a 50 Hz, 0.5 mT field. Cell surface morphology (scanning electron microscopy), cell growth characteristics (growth curves and cell cycle phase distribution), and cell death (necrosis and apoptosis) were also examined. The results demonstrate that no variations in surface morphology and cell death occurred between control and exposed cells in both MG-63 and Saos-2 cells, while significant changes were noted in cell growth and fibronectin and CD44 expression in MG-63 cells. The results are discussed in view of the important role that CAMs play in controlling various cancer cell functions, particularly proliferation and metastasis.

Original languageEnglish
Pages (from-to)327-338
Number of pages12
JournalBioelectromagnetics
Volume24
Issue number5
DOIs
Publication statusPublished - Jul 2003

Keywords

  • Apoptosis
  • Bone cells
  • Cancer cells
  • Cell adhesion molecules
  • Cell cycle phases
  • ELF

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics

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