Effects of a ceramic biomaterial on immune modulatory properties and differentiation potential of human mesenchymal stromal cells of different origin

Giulio Bassi, Fabien Guilloton, Cedric Menard, Mariano Di Trapani, Frederic Deschaseaux, Luc Sensebé, Hubert Schrezenmeier, Rosaria Giordano, Philippe Bourin, Massimo Dominici, Karin Tarte, Mauro Krampera

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to assess the immune modulatory properties of human mesenchymal stromal cells obtained from bone marrow (BM-MSCs), fat (ASCs), and cord blood (CB-MSCs) in the presence of a hydroxyapatite and tricalcium-phosphate (HA/TCP) biomaterial as a scaffold for MSC delivery. In resting conditions, a short-term culture with HA/TCP did not modulate the anti-apoptotic and suppressive features of the various MSC types toward T, B, and NK cells; in addition, when primed with inflammatory cytokines, MSCs similarly increased their suppressive capacities in the presence or absence of HA/TCP. The long-term culture of BM-MSCs with HA/TCP induced an osteoblast-like phenotype with upregulation of OSTERIX and OSTEOCALCIN, similar to what was obtained with dexamethasone and, to a higher extent, with bone morphogenetic protein 4 (BMP-4) treatment. MSC-derived osteoblasts did not trigger immune cell activation, but were less efficient than undifferentiated MSCs in inhibiting stimulated T and NK cells. Interestingly, their suppressive machinery included not only the activation of indoleamine-2,3 dioxygenase (IDO), which plays a central role in T-cell inhibition, but also cyclooxygenase-2 (COX-2) that was not significantly involved in the immune modulatory effect of human undifferentiated MSCs. Since COX-2 is significantly involved in bone healing, its induction by HA/TCP could also contribute to the therapeutic activity of MSCs for bone tissue engineering.

Original languageEnglish
Pages (from-to)767-781
Number of pages15
JournalTissue Engineering - Part A
Volume21
Issue number3-4
DOIs
Publication statusPublished - Feb 1 2015

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Ceramics
Biocompatible Materials
Durapatite
Mesenchymal Stromal Cells
Hydroxyapatite
Biomaterials
Phosphates
Bone
Osteoblasts
Cyclooxygenase 2
T-Lymphocytes
Natural Killer Cells
Chemical activation
Bone Morphogenetic Protein 4
Indoleamine-Pyrrole 2,3,-Dioxygenase
Bone and Bones
T-cells
Tissue Engineering
Oils and fats
Fetal Blood

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Effects of a ceramic biomaterial on immune modulatory properties and differentiation potential of human mesenchymal stromal cells of different origin. / Bassi, Giulio; Guilloton, Fabien; Menard, Cedric; Di Trapani, Mariano; Deschaseaux, Frederic; Sensebé, Luc; Schrezenmeier, Hubert; Giordano, Rosaria; Bourin, Philippe; Dominici, Massimo; Tarte, Karin; Krampera, Mauro.

In: Tissue Engineering - Part A, Vol. 21, No. 3-4, 01.02.2015, p. 767-781.

Research output: Contribution to journalArticle

Bassi, G, Guilloton, F, Menard, C, Di Trapani, M, Deschaseaux, F, Sensebé, L, Schrezenmeier, H, Giordano, R, Bourin, P, Dominici, M, Tarte, K & Krampera, M 2015, 'Effects of a ceramic biomaterial on immune modulatory properties and differentiation potential of human mesenchymal stromal cells of different origin', Tissue Engineering - Part A, vol. 21, no. 3-4, pp. 767-781. https://doi.org/10.1089/ten.tea.2014.0269
Bassi, Giulio ; Guilloton, Fabien ; Menard, Cedric ; Di Trapani, Mariano ; Deschaseaux, Frederic ; Sensebé, Luc ; Schrezenmeier, Hubert ; Giordano, Rosaria ; Bourin, Philippe ; Dominici, Massimo ; Tarte, Karin ; Krampera, Mauro. / Effects of a ceramic biomaterial on immune modulatory properties and differentiation potential of human mesenchymal stromal cells of different origin. In: Tissue Engineering - Part A. 2015 ; Vol. 21, No. 3-4. pp. 767-781.
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