Effects of a low dose of bentazon on spermatogenesis of mice exposed during foetal, postnatal and adult life

Silvia Garagna, Chiara Vasco, Valeria Merico, Antonio Esposito, Maurizio Zuccotti, Carlo Alberto Redi

Research output: Contribution to journalArticle

Abstract

Bentazon is a herbicide used to control many broadleaf weeds and sedges. Its use has improved rice production in paddy fields in Northern Italy, but as a negative consequence it is found in groundwater, the major source of drinking water. To determine whether low doses of bentazon affect spermatogenesis, it was dissolved in water at the concentration of 30 μg/L. Bentazon was administered through drinking water to: (1) adult mice for 100 days and (2) mice exposed in utero, during lactation and for 100 days after birth. The histopathological analysis of testes of treated animals showed that the frequency of defective tubules was comparable to that found in control groups. The cell associations of the 12 stages of the seminiferous epithelium were correct as well as the architecture of the epithelium. The spermatocytes/ spermatids ratio was the same as in controls. However, the frequency of stages VII, IX and XII of the cycle of the seminiferous epithelium of adult mice and of stages I, III and VII of mice exposed in utero and for 100 days after birth was different when compared to that of control mice. Sperm number and morphology were not affected by the treatment. The potential genotoxic effects were evaluated on spermatozoa (Comet assay), in pachytene spermatocytes (analysis of the synaptonemal complex) and in bone marrow cells (frequency of micronuclei). None of these analyses evidenced genotoxic effects of bentazon. Although our results show that the administration of a low dose of bentazon does not impair spermatogenesis, we found alterations of the frequency of some stages of the cycle of the seminiferous epithelium in both experimental groups.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalToxicology
Volume212
Issue number2-3
DOIs
Publication statusPublished - Sep 1 2005

Fingerprint

Spermatogenesis
Seminiferous Epithelium
Spermatocytes
Drinking Water
Parturition
Synaptonemal Complex
Comet Assay
Sperm Count
Spermatids
Groundwater
Herbicides
Lactation
Bone Marrow Cells
Italy
Spermatozoa
Testis
Assays
Bone
Animals
Epithelium

Keywords

  • Bentazon
  • Comet assay
  • Micronuclei
  • Mouse
  • Sperm morphology
  • Spermatogenesis
  • Synaptonemal complex

ASJC Scopus subject areas

  • Toxicology

Cite this

Garagna, S., Vasco, C., Merico, V., Esposito, A., Zuccotti, M., & Redi, C. A. (2005). Effects of a low dose of bentazon on spermatogenesis of mice exposed during foetal, postnatal and adult life. Toxicology, 212(2-3), 165-174. https://doi.org/10.1016/j.tox.2005.04.017

Effects of a low dose of bentazon on spermatogenesis of mice exposed during foetal, postnatal and adult life. / Garagna, Silvia; Vasco, Chiara; Merico, Valeria; Esposito, Antonio; Zuccotti, Maurizio; Redi, Carlo Alberto.

In: Toxicology, Vol. 212, No. 2-3, 01.09.2005, p. 165-174.

Research output: Contribution to journalArticle

Garagna, S, Vasco, C, Merico, V, Esposito, A, Zuccotti, M & Redi, CA 2005, 'Effects of a low dose of bentazon on spermatogenesis of mice exposed during foetal, postnatal and adult life', Toxicology, vol. 212, no. 2-3, pp. 165-174. https://doi.org/10.1016/j.tox.2005.04.017
Garagna, Silvia ; Vasco, Chiara ; Merico, Valeria ; Esposito, Antonio ; Zuccotti, Maurizio ; Redi, Carlo Alberto. / Effects of a low dose of bentazon on spermatogenesis of mice exposed during foetal, postnatal and adult life. In: Toxicology. 2005 ; Vol. 212, No. 2-3. pp. 165-174.
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