TY - JOUR
T1 - Effects of age-related loss of P/Q-type calcium channels in a mice model of peripheral nerve injury
AU - Marinelli, Sara
AU - Eleuteri, Cecilia
AU - Vacca, Valentina
AU - Strimpakos, Georgios
AU - Mattei, Elisabetta
AU - Severini, Cinzia
AU - Pavone, Flaminia
AU - Luvisetto, Siro
PY - 2015/1/1
Y1 - 2015/1/1
N2 - We analyzed the role of P/Q-type calcium channels in sciatic nerve regeneration after lesion induced by chronic constriction injury (CCI) in heterozygous null mutant mice lacking the CaV2.1α1 subunit of these channels (Cacna1a+/-). Compared with wild type, Cacna1a+/- mice showed an initial reduction of the CCI-induced allodynia, indicating a reduced pain perception, but they also evidenced a lack of recovery over time, with atrophy of the injured hindpaw still present 3months after CCI when wild-type mice fully recovered. In parallel, Cacna1a+/- mice exhibited an early onset of age-dependent loss of P/Q-type channels, which can be responsible for the lack of functional recovery. Moreover, Cacna1a+/- mice showed an early age-dependent reduction of muscular strength, as well as of Schwann cells proliferation and sciatic nerve remyelination. This study demonstrates the important role played by P/Q-type channels in recovery from nerve injury and has important implications for the knowledge of age-related processes.
AB - We analyzed the role of P/Q-type calcium channels in sciatic nerve regeneration after lesion induced by chronic constriction injury (CCI) in heterozygous null mutant mice lacking the CaV2.1α1 subunit of these channels (Cacna1a+/-). Compared with wild type, Cacna1a+/- mice showed an initial reduction of the CCI-induced allodynia, indicating a reduced pain perception, but they also evidenced a lack of recovery over time, with atrophy of the injured hindpaw still present 3months after CCI when wild-type mice fully recovered. In parallel, Cacna1a+/- mice exhibited an early onset of age-dependent loss of P/Q-type channels, which can be responsible for the lack of functional recovery. Moreover, Cacna1a+/- mice showed an early age-dependent reduction of muscular strength, as well as of Schwann cells proliferation and sciatic nerve remyelination. This study demonstrates the important role played by P/Q-type channels in recovery from nerve injury and has important implications for the knowledge of age-related processes.
KW - Allodynia
KW - Cacna1a
KW - Muscle
KW - Nerve regeneration
KW - P/Q-type channels
KW - Schwann cells
UR - http://www.scopus.com/inward/record.url?scp=84920575658&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920575658&partnerID=8YFLogxK
U2 - 10.1016/j.neurobiolaging.2014.07.025
DO - 10.1016/j.neurobiolaging.2014.07.025
M3 - Article
C2 - 25150573
AN - SCOPUS:84920575658
VL - 36
SP - 352
EP - 364
JO - Neurobiology of Aging
JF - Neurobiology of Aging
SN - 0197-4580
IS - 1
ER -