Amylin has been reported to have bone-conserving effects. In the present study we evaluated the possible activity of the peptide on human osteoblast-like (hOB) cells in primary culture. Amylin between 10 -9 and 10 -6 M, dose-dependently stimulated cell proliferation with a maximal effect (200%) at 10 -6 M. In addition, amylin increased osteocalcin production when hOB cells were exposed to 1,25(OH) 2D 3 (10 -8 M) but there was a nonsignificant upward trend on alkaline phosphatase activity. The present results suggest that amylin could be included among the group of peptides endowed with osteogenic activity.
- Alkaline phosphatase
- Osteoblast-like cells
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience