Effects of atrial natriuretic factor on human platelet function

Raffaele De Caterina, Massimo Volpe, Steven A. Atlas, Babette B. Weksler

Research output: Contribution to journalArticlepeer-review


We examined the hypothesis that atrial natriuretic factor (ANF), a substance with known vasorelaxant activities, shares with other vasodilators the property of inhibiting platelet function. Aggregation of citrated platelet-rich plasma (PRP) from 23 healthy volunteers induced by ADP, adrenaline, arachidonic acid, collagen, γ-thrombin, the endoperoxide analogue U-44069, serotonin, the calcium ionophore A-23187 or platelet aggregating factor was measured after incubation of PRP with ANF for 3 minutes at concentrations of 4 × 10-9, 4 × 10-8 and 4 × 10-7M or vehicle as control. ANF decreased ADP-induced aggregation significantly (P-7M: 60.0±17.1%, mean±S.D., n=39) by a selective inhibitory effect on the secondary wave; neither aggregation by all other agents tested nor thromboxane B2 generation induced by ADP and adrenaline was altered by incubation with ANF. Although ANF thus has detectable effects on ADP-induced platelet aggregation in vitro, these data suggest that ANF is unlikely to be a physiologically significant modulator of platelet function.

Original languageEnglish
Pages (from-to)1395-1402
Number of pages8
JournalLife Sciences
Issue number15
Publication statusPublished - Oct 14 1985

ASJC Scopus subject areas

  • Pharmacology


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