TY - JOUR
T1 - Effects of atrial natriuretic factor on human platelet function
AU - De Caterina, Raffaele
AU - Volpe, Massimo
AU - Atlas, Steven A.
AU - Weksler, Babette B.
PY - 1985/10/14
Y1 - 1985/10/14
N2 - We examined the hypothesis that atrial natriuretic factor (ANF), a substance with known vasorelaxant activities, shares with other vasodilators the property of inhibiting platelet function. Aggregation of citrated platelet-rich plasma (PRP) from 23 healthy volunteers induced by ADP, adrenaline, arachidonic acid, collagen, γ-thrombin, the endoperoxide analogue U-44069, serotonin, the calcium ionophore A-23187 or platelet aggregating factor was measured after incubation of PRP with ANF for 3 minutes at concentrations of 4 × 10-9, 4 × 10-8 and 4 × 10-7M or vehicle as control. ANF decreased ADP-induced aggregation significantly (P-7M: 60.0±17.1%, mean±S.D., n=39) by a selective inhibitory effect on the secondary wave; neither aggregation by all other agents tested nor thromboxane B2 generation induced by ADP and adrenaline was altered by incubation with ANF. Although ANF thus has detectable effects on ADP-induced platelet aggregation in vitro, these data suggest that ANF is unlikely to be a physiologically significant modulator of platelet function.
AB - We examined the hypothesis that atrial natriuretic factor (ANF), a substance with known vasorelaxant activities, shares with other vasodilators the property of inhibiting platelet function. Aggregation of citrated platelet-rich plasma (PRP) from 23 healthy volunteers induced by ADP, adrenaline, arachidonic acid, collagen, γ-thrombin, the endoperoxide analogue U-44069, serotonin, the calcium ionophore A-23187 or platelet aggregating factor was measured after incubation of PRP with ANF for 3 minutes at concentrations of 4 × 10-9, 4 × 10-8 and 4 × 10-7M or vehicle as control. ANF decreased ADP-induced aggregation significantly (P-7M: 60.0±17.1%, mean±S.D., n=39) by a selective inhibitory effect on the secondary wave; neither aggregation by all other agents tested nor thromboxane B2 generation induced by ADP and adrenaline was altered by incubation with ANF. Although ANF thus has detectable effects on ADP-induced platelet aggregation in vitro, these data suggest that ANF is unlikely to be a physiologically significant modulator of platelet function.
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U2 - 10.1016/0024-3205(85)90078-5
DO - 10.1016/0024-3205(85)90078-5
M3 - Article
C2 - 2931566
AN - SCOPUS:0021840463
VL - 37
SP - 1395
EP - 1402
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 15
ER -