Effects of bevacizumab on neuronal viability of retinal ganglion cells in rats

Maria Rosaria Romano, Francesca Biagioni, Gianluca Besozzi, Albino Carrizzo, Carmine Vecchione, Francesco Fornai, Marcello Diego Lograno

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the effects of single and repeated intravitreal injections of bevacizumab on various retinal layers focusing more on retinal ganglion cells (RGCs) in healthy rats. Male Wistar rats were treated with intravitreal injection of bevacizimab (4 μL) within right eye. Left eyes were injected with the same volume of balanced salt solution (BSS) and used as control. Ten rats received a single intravitreal injection and ten rats had three injections, with seven days time interval. Histological and immunohistochemical evaluations and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay were performed in order to find out if some degree of apoptosis could occur on RGCs. Histological and immunohistochemical analyses showed that bevacizumab induces neuronal loss compared to control eyes, after multiple injections. RGCs apoptosis after multiple treatments was demonstrated to occur by TUNEL, Annexin V and Bax assays. The loss of ganglion cells following repeated injections was confirmed and quantified by the decrease in RGC specific protein Brn3a measured by western blotting in ten additional rats. The present results need to be considered when multiple intravitreal injection of bevacizumab are performed to treat retinal diseases.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalBrain Research
Volume1478
DOIs
Publication statusPublished - Oct 10 2012

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Keywords

  • Age-related macular degeneration
  • Apoptosis
  • Bevacizumab
  • Retinal ganglion cell (RGC)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

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