Abstract
Background: Bicalutamide (BIC) is an alternative treatment to castration for advanced prostate cancer. Breast events are common adverse effects which can be effectively prevented by the concurrent administration of tamoxifen, a selective estrogen receptor modulator. Materials and Methods: We investigated the effects of BIC, 4-hydroxy Tamoxifen (4OHT), the active metabolite of tamoxifen, and their combination on the expression of a panel of genes implicated in prostate cancer development and progression in LNCaP cells stimulated with dihydrotestosterone. Results: Our findings confirm the anti-proliferative activity of BIC on LNCaP cell growth but also show the down-regulating function of this anti-androgen on the expression of genes involved in tumor proliferation and invasion [cyclins, caspases, epidermal growth factor (EGF)]. The combination with 4OHT exerts a synergistic effect on the downregulation of some genes involved in prostate cancer progression. Conclusion: The observation that the expression of several genes [such as B-cell lymphoma-2 (BCL2), myelocytomatosis oncogene (MYC), caspases] is modulated midly-to-moderately, after 4OHT addition suggests that this combined approach in the clinical setting should be further investigated through appropriate trials.
| Original language | English |
|---|---|
| Pages (from-to) | 5323-5329 |
| Number of pages | 7 |
| Journal | Anticancer Research |
| Volume | 32 |
| Issue number | 12 |
| Publication status | Published - Dec 2012 |
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Keywords
- 4OH-Tamoxifen
- Bicalutamide
- LNCaP cells
- Prostate cancer
- Quantitative RT-PCR
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Effects of bicalutamide and 4OH-tamoxifen on androgen-regulated gene expression in the LNCaP cell line. / Mangerini, Rosa; Argellati, Francesca; Pfeffer, Ulrich; Boccardo, Francesco.
In: Anticancer Research, Vol. 32, No. 12, 12.2012, p. 5323-5329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of bicalutamide and 4OH-tamoxifen on androgen-regulated gene expression in the LNCaP cell line
AU - Mangerini, Rosa
AU - Argellati, Francesca
AU - Pfeffer, Ulrich
AU - Boccardo, Francesco
PY - 2012/12
Y1 - 2012/12
N2 - Background: Bicalutamide (BIC) is an alternative treatment to castration for advanced prostate cancer. Breast events are common adverse effects which can be effectively prevented by the concurrent administration of tamoxifen, a selective estrogen receptor modulator. Materials and Methods: We investigated the effects of BIC, 4-hydroxy Tamoxifen (4OHT), the active metabolite of tamoxifen, and their combination on the expression of a panel of genes implicated in prostate cancer development and progression in LNCaP cells stimulated with dihydrotestosterone. Results: Our findings confirm the anti-proliferative activity of BIC on LNCaP cell growth but also show the down-regulating function of this anti-androgen on the expression of genes involved in tumor proliferation and invasion [cyclins, caspases, epidermal growth factor (EGF)]. The combination with 4OHT exerts a synergistic effect on the downregulation of some genes involved in prostate cancer progression. Conclusion: The observation that the expression of several genes [such as B-cell lymphoma-2 (BCL2), myelocytomatosis oncogene (MYC), caspases] is modulated midly-to-moderately, after 4OHT addition suggests that this combined approach in the clinical setting should be further investigated through appropriate trials.
AB - Background: Bicalutamide (BIC) is an alternative treatment to castration for advanced prostate cancer. Breast events are common adverse effects which can be effectively prevented by the concurrent administration of tamoxifen, a selective estrogen receptor modulator. Materials and Methods: We investigated the effects of BIC, 4-hydroxy Tamoxifen (4OHT), the active metabolite of tamoxifen, and their combination on the expression of a panel of genes implicated in prostate cancer development and progression in LNCaP cells stimulated with dihydrotestosterone. Results: Our findings confirm the anti-proliferative activity of BIC on LNCaP cell growth but also show the down-regulating function of this anti-androgen on the expression of genes involved in tumor proliferation and invasion [cyclins, caspases, epidermal growth factor (EGF)]. The combination with 4OHT exerts a synergistic effect on the downregulation of some genes involved in prostate cancer progression. Conclusion: The observation that the expression of several genes [such as B-cell lymphoma-2 (BCL2), myelocytomatosis oncogene (MYC), caspases] is modulated midly-to-moderately, after 4OHT addition suggests that this combined approach in the clinical setting should be further investigated through appropriate trials.
KW - 4OH-Tamoxifen
KW - Bicalutamide
KW - LNCaP cells
KW - Prostate cancer
KW - Quantitative RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=84872570178&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872570178&partnerID=8YFLogxK
M3 - Article
C2 - 23225433
AN - SCOPUS:84872570178
VL - 32
SP - 5323
EP - 5329
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 12
ER -