Effects of blood volume restitution following a portal hypertensive-related bleeding in anesthetized cirrhotic rats

B. Castañeda, J. Morales, R. Lionetti, E. Moitinho, V. Andreu, S. Pérez-del-Pulgar, P. Pizcueta, J. Rodés, J. Bosch

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the influence of different strategies of blood volume restitution in the outcome of portal hypertension-related bleeding in anesthetized cirrhotic rats. Gastrointestinal hemorrhage was induced by sectioning a first order branch of the ileocolic vein in 38 cirrhotic rats (common bile duct ligation and occlusion). The subsequent hypovolemic shock was treated with no transfusion (n = 17), moderate transfusion (50% of expected blood loss, 5 mL, n = 11), and total transfusion (100% of expected blood loss, 10 mL, n = 10). At the end of the blood transfusion period (minute 15), mean arterial pressure (MAP) partially recovered in rats receiving moderate transfusion or no transfusion but decreased in the 10-mL transfusion group (↓ 12 ± 43%, P <.05 vs. no transfusion and 5 mL transfusion). After transfusion, groups given no or 5 mL transfusion remained hemodynamically stable. However, rats receiving 10 mL transfusion continued to deteriorate with persistent bleeding and progressive fall in MAP (↓ 65 ± 12%; P <.05 vs. no transfusion and 5 mL transfusion). Collected blood loss was significantly greater in the 10-mL group (20.0 ± 1.5 g) than in groups given 5 mL (15.9 ± 2.8 g; P <.05) or no transfusion (13.2 ± 2.1 g; P <.05 vs. 10 mL and 5 mL transfusion). Survival in the no transfusion group was 47%. Rats given 5-mL transfusion had 64% survival. The worst survival was observed in the 10-mL transfusion group (0% survival; P <.05). We concluded that a transfusion policy aimed at completely replacing blood loss worsens the magnitude of bleeding and mortality from portal hypertensive-related bleeding in cirrhotic rats. On the contrary, moderate blood transfusion allowed hemodynamic stabilization and increased survival.

Original languageEnglish
Pages (from-to)821-825
Number of pages5
JournalHepatology
Volume33
Issue number4
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Hepatology

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    Castañeda, B., Morales, J., Lionetti, R., Moitinho, E., Andreu, V., Pérez-del-Pulgar, S., Pizcueta, P., Rodés, J., & Bosch, J. (2001). Effects of blood volume restitution following a portal hypertensive-related bleeding in anesthetized cirrhotic rats. Hepatology, 33(4), 821-825. https://doi.org/10.1053/jhep.2001.23437