Effects of cadmium on lymphocyte proliferation and macrophage activation in catfish,Ictalurus melas

Vincenzo Albergoni, Antonella Viola

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of cadmium (Cd) (in vitroandin vivo) on phytohaemoagglutinin(PHA)- or lipopolysaccharide(LPS)-induced lymphocyte proliferation were investigated in the catfish,Ictalurus melas. The effects of Cd on macrophage activation by Concanavallina A orSaccharomyces cerevisiae, as measured by superoxide (O2-) production, were investigated. Dose-independent (except at 2 μmCd) inhibition of T cell stimulation and dose-dependent inhibition of B cell stimulation, by lymphocytes exposedin vitroto 2-40 μmCd, was found.In vivo, 20 μg l-1Cd inhibited both PHA- and LPS-induced lymphocyte proliferation, at 4 days of exposure and throughout the duration of the experiment. During the first week of exposure, Cd significantly decreased spontaneous3H-methyl-thymidine uptake from unstimulated lymphocytes; however, 15 days after exposure unstimulated cells recovered their basal level of spontaneous thymidine uptake.In vitro2-40 μmCd enhanced O2-production by activated macrophages, while the addition of 50-100 μmCd had no significant effects.

Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalFish and Shellfish Immunology
Volume5
Issue number4
DOIs
Publication statusPublished - May 1995

Keywords

  • cadmium
  • catfish
  • lymphocytes
  • macrophages

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

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