Effects of calcium on Na+, K+-ATPase isolated from human placenta

L. Mazzanti, R. A. Rabini, A. M. Cugini, G. Zolese

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It has been reported that Ca2+ can be either stimulatory or inhibitory of the activity of Na+,K+-ATPase obtained from different sources. The aim of the present work was to study the effect of increasing concentrations of Ca2+ on Na+,K+-ATPase purified from human placenta. The temperature dependence of Na+,K+-ATPase activity and of spectral parameters of a spin label paramagnetic maleimide derivative (MSL, 2,2,6,6-tetramethylpiperidin-1-oxyl-4-maleimide) were also investigated in absence and in presence of 2 mM Ca2+. Ca2+ affects positively the enzymatic activity and this effect is evident at all temperature tested. Maleimide spectral parameters are not affected by the presence of 2 mM Ca2+ showing that the microenvironments of SH groups are not modified following ion interactions.

Original languageEnglish
Pages (from-to)847-852
Number of pages6
JournalBiochemistry International
Issue number5
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Biochemistry


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