Effects of calcium on Na+, K+-ATPase isolated from human placenta

L. Mazzanti, R. A. Rabini, A. M. Cugini, G. Zolese

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been reported that Ca2+ can be either stimulatory or inhibitory of the activity of Na+,K+-ATPase obtained from different sources. The aim of the present work was to study the effect of increasing concentrations of Ca2+ on Na+,K+-ATPase purified from human placenta. The temperature dependence of Na+,K+-ATPase activity and of spectral parameters of a spin label paramagnetic maleimide derivative (MSL, 2,2,6,6-tetramethylpiperidin-1-oxyl-4-maleimide) were also investigated in absence and in presence of 2 mM Ca2+. Ca2+ affects positively the enzymatic activity and this effect is evident at all temperature tested. Maleimide spectral parameters are not affected by the presence of 2 mM Ca2+ showing that the microenvironments of SH groups are not modified following ion interactions.

Original languageEnglish
Pages (from-to)847-852
Number of pages6
JournalBiochemistry International
Volume27
Issue number5
Publication statusPublished - 1992

Fingerprint

Placenta
Adenosine Triphosphatases
Calcium
Spin Labels
Temperature
Ions
Derivatives
maleimide
sodium-translocating ATPase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effects of calcium on Na+, K+-ATPase isolated from human placenta. / Mazzanti, L.; Rabini, R. A.; Cugini, A. M.; Zolese, G.

In: Biochemistry International, Vol. 27, No. 5, 1992, p. 847-852.

Research output: Contribution to journalArticle

Mazzanti, L, Rabini, RA, Cugini, AM & Zolese, G 1992, 'Effects of calcium on Na+, K+-ATPase isolated from human placenta', Biochemistry International, vol. 27, no. 5, pp. 847-852.
Mazzanti, L. ; Rabini, R. A. ; Cugini, A. M. ; Zolese, G. / Effects of calcium on Na+, K+-ATPase isolated from human placenta. In: Biochemistry International. 1992 ; Vol. 27, No. 5. pp. 847-852.
@article{62d20d03b06f424f93eea389d68ec629,
title = "Effects of calcium on Na+, K+-ATPase isolated from human placenta",
abstract = "It has been reported that Ca2+ can be either stimulatory or inhibitory of the activity of Na+,K+-ATPase obtained from different sources. The aim of the present work was to study the effect of increasing concentrations of Ca2+ on Na+,K+-ATPase purified from human placenta. The temperature dependence of Na+,K+-ATPase activity and of spectral parameters of a spin label paramagnetic maleimide derivative (MSL, 2,2,6,6-tetramethylpiperidin-1-oxyl-4-maleimide) were also investigated in absence and in presence of 2 mM Ca2+. Ca2+ affects positively the enzymatic activity and this effect is evident at all temperature tested. Maleimide spectral parameters are not affected by the presence of 2 mM Ca2+ showing that the microenvironments of SH groups are not modified following ion interactions.",
author = "L. Mazzanti and Rabini, {R. A.} and Cugini, {A. M.} and G. Zolese",
year = "1992",
language = "English",
volume = "27",
pages = "847--852",
journal = "Biochemistry and Molecular Biology International",
issn = "1039-9712",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Effects of calcium on Na+, K+-ATPase isolated from human placenta

AU - Mazzanti, L.

AU - Rabini, R. A.

AU - Cugini, A. M.

AU - Zolese, G.

PY - 1992

Y1 - 1992

N2 - It has been reported that Ca2+ can be either stimulatory or inhibitory of the activity of Na+,K+-ATPase obtained from different sources. The aim of the present work was to study the effect of increasing concentrations of Ca2+ on Na+,K+-ATPase purified from human placenta. The temperature dependence of Na+,K+-ATPase activity and of spectral parameters of a spin label paramagnetic maleimide derivative (MSL, 2,2,6,6-tetramethylpiperidin-1-oxyl-4-maleimide) were also investigated in absence and in presence of 2 mM Ca2+. Ca2+ affects positively the enzymatic activity and this effect is evident at all temperature tested. Maleimide spectral parameters are not affected by the presence of 2 mM Ca2+ showing that the microenvironments of SH groups are not modified following ion interactions.

AB - It has been reported that Ca2+ can be either stimulatory or inhibitory of the activity of Na+,K+-ATPase obtained from different sources. The aim of the present work was to study the effect of increasing concentrations of Ca2+ on Na+,K+-ATPase purified from human placenta. The temperature dependence of Na+,K+-ATPase activity and of spectral parameters of a spin label paramagnetic maleimide derivative (MSL, 2,2,6,6-tetramethylpiperidin-1-oxyl-4-maleimide) were also investigated in absence and in presence of 2 mM Ca2+. Ca2+ affects positively the enzymatic activity and this effect is evident at all temperature tested. Maleimide spectral parameters are not affected by the presence of 2 mM Ca2+ showing that the microenvironments of SH groups are not modified following ion interactions.

UR - http://www.scopus.com/inward/record.url?scp=0026709357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026709357&partnerID=8YFLogxK

M3 - Article

C2 - 1329752

AN - SCOPUS:0026709357

VL - 27

SP - 847

EP - 852

JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

SN - 1039-9712

IS - 5

ER -