TY - JOUR
T1 - Effects of cholinergic stimulation with pyridostigmine bromide on chronic chagasic cardiomyopathic mice
AU - Cuba, Marília Beatriz De
AU - Ribeiro Machado, Marcus Paulo
AU - Farnesi, Thais Soares
AU - Alves, Angelica Cristina
AU - Martins, Livia Alves
AU - Oliveira, Lucas Felipe De
AU - Capitelli, Caroline Santos
AU - Leite, Camila Ferreira
AU - Vinícius Silva, Marcos
AU - Machado, Juliana Reis
AU - Kappel, Henrique Borges
AU - Sales De Campos, Helioswilton
AU - Paiva, Luciano
AU - Da Silva Gomes, Natália Lins
AU - Guimarães Faleiros, Ana Carolina
AU - De Paoli De Carvalho Britto, Constança Felicia
AU - Savino, Wilson
AU - Moreira, Otacílio Cruz
AU - Rodrigues, Virmondes
AU - Montano, Nicola
AU - Lages-Silva, Eliane
AU - Ramirez, Luis Eduardo
AU - Dias Da Silva, Valdo Jose
PY - 2014
Y1 - 2014
N2 - The aim of the present study was to assess the effects of an anticholinesterase agent, pyridostigmine bromide (Pyrido), on experimental chronic Chagas heart disease in mice. To this end, male C57BL/6J mice noninfected (control:Con) or chronically infected (5 months) with Trypanosoma cruzi (chagasic:Chg) were treated or not (NT) with Pyrido for one month. At the end of this period, electrocardiogram (ECG); cardiac autonomic function; heart histopathology; serum cytokines; and the presence of blood and tissue parasites by means of immunohistochemistry and PCR were assessed. In NT-Chg mice, significant changes in the electrocardiographic, autonomic, and cardiac histopathological profiles were observed confirming a chronic inflammatory response. Treatment with Pyrido in Chagasic mice caused a significant reduction of myocardial inflammatory infiltration, fibrosis, and hypertrophy, which was accompanied by a decrease in serum levels of IFNγ with no change in IL-10 levels, suggesting a shift of immune response toward an anti-inflammatory profile. Lower nondifferent numbers of parasite DNA copies were observed in both treated and nontreated chagasic mice. In conclusion, our findings confirm the marked neuroimmunomodulatory role played by the parasympathetic autonomic nervous system in the evolution of the inflammatory-immune response to T. cruzi during experimental chronic Chagas heart disease in mice.
AB - The aim of the present study was to assess the effects of an anticholinesterase agent, pyridostigmine bromide (Pyrido), on experimental chronic Chagas heart disease in mice. To this end, male C57BL/6J mice noninfected (control:Con) or chronically infected (5 months) with Trypanosoma cruzi (chagasic:Chg) were treated or not (NT) with Pyrido for one month. At the end of this period, electrocardiogram (ECG); cardiac autonomic function; heart histopathology; serum cytokines; and the presence of blood and tissue parasites by means of immunohistochemistry and PCR were assessed. In NT-Chg mice, significant changes in the electrocardiographic, autonomic, and cardiac histopathological profiles were observed confirming a chronic inflammatory response. Treatment with Pyrido in Chagasic mice caused a significant reduction of myocardial inflammatory infiltration, fibrosis, and hypertrophy, which was accompanied by a decrease in serum levels of IFNγ with no change in IL-10 levels, suggesting a shift of immune response toward an anti-inflammatory profile. Lower nondifferent numbers of parasite DNA copies were observed in both treated and nontreated chagasic mice. In conclusion, our findings confirm the marked neuroimmunomodulatory role played by the parasympathetic autonomic nervous system in the evolution of the inflammatory-immune response to T. cruzi during experimental chronic Chagas heart disease in mice.
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U2 - 10.1155/2014/475946
DO - 10.1155/2014/475946
M3 - Article
C2 - 25221388
AN - SCOPUS:84930010401
VL - 2014
JO - Mediators of Inflammation
JF - Mediators of Inflammation
SN - 0962-9351
M1 - 475946
ER -