Abstract
Altered endothelium-dependent vasodilation has been observed in congestive heart failure (CHF), a disease characterized by a sustained adrenergic activation. The purpose of our study was to test the hypothesis that chronically elevated catecholamines influence the nitric oxide (NO) pathway in the human endothelium. Human umbilical vein endothelial cells (HUVEC) were exposed for 7 days to a concentration of noradrenaline (NA,1 ng/mL) similar to that found in the blood of patients with CHE Kinetics of endothelial constitutive NO synthase (ecNOS) and inducible NO synthase (iNOS) activity, measured by [3H]L arginine to [3H]L-citrulline conversion, and protein expression of ecNOS and iNOS, assessed by Western blot analysis, were unaffected by chronic NA treatment. Furthermore, no changes in subcellular fraction-associated ecNOS were found; this indirectly shows that chronic NA did not cause phosphorylation of the enzyme. Moreover, [3H]L-arginine transport through the plasma membrane was conserved in chronically NA-treated cells. The data demonstrate that prolonged in vitro exposure to pathologic CHF-like NA does not affect the L-arginine: NO pathway in human endothelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 250-256 |
| Number of pages | 7 |
| Journal | Basic Research in Cardiology |
| Volume | 93 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1998 |
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Keywords
- Endothelial cells
- Nitric oxide
- Nitric oxide synthase
- Noradrenaline
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Effects of chronic noradrenaline on the nitric oxide pathway in human endothelial cells. / Bachetti, T.; Comini, L.; Agnoletti, L.; Pedersini, P.; Gaia, G.; Cargnoni, A.; Bellet, M.; Curello, S.; Ferrari, R.
In: Basic Research in Cardiology, Vol. 93, No. 4, 08.1998, p. 250-256.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of chronic noradrenaline on the nitric oxide pathway in human endothelial cells
AU - Bachetti, T.
AU - Comini, L.
AU - Agnoletti, L.
AU - Pedersini, P.
AU - Gaia, G.
AU - Cargnoni, A.
AU - Bellet, M.
AU - Curello, S.
AU - Ferrari, R.
PY - 1998/8
Y1 - 1998/8
N2 - Altered endothelium-dependent vasodilation has been observed in congestive heart failure (CHF), a disease characterized by a sustained adrenergic activation. The purpose of our study was to test the hypothesis that chronically elevated catecholamines influence the nitric oxide (NO) pathway in the human endothelium. Human umbilical vein endothelial cells (HUVEC) were exposed for 7 days to a concentration of noradrenaline (NA,1 ng/mL) similar to that found in the blood of patients with CHE Kinetics of endothelial constitutive NO synthase (ecNOS) and inducible NO synthase (iNOS) activity, measured by [3H]L arginine to [3H]L-citrulline conversion, and protein expression of ecNOS and iNOS, assessed by Western blot analysis, were unaffected by chronic NA treatment. Furthermore, no changes in subcellular fraction-associated ecNOS were found; this indirectly shows that chronic NA did not cause phosphorylation of the enzyme. Moreover, [3H]L-arginine transport through the plasma membrane was conserved in chronically NA-treated cells. The data demonstrate that prolonged in vitro exposure to pathologic CHF-like NA does not affect the L-arginine: NO pathway in human endothelial cells.
AB - Altered endothelium-dependent vasodilation has been observed in congestive heart failure (CHF), a disease characterized by a sustained adrenergic activation. The purpose of our study was to test the hypothesis that chronically elevated catecholamines influence the nitric oxide (NO) pathway in the human endothelium. Human umbilical vein endothelial cells (HUVEC) were exposed for 7 days to a concentration of noradrenaline (NA,1 ng/mL) similar to that found in the blood of patients with CHE Kinetics of endothelial constitutive NO synthase (ecNOS) and inducible NO synthase (iNOS) activity, measured by [3H]L arginine to [3H]L-citrulline conversion, and protein expression of ecNOS and iNOS, assessed by Western blot analysis, were unaffected by chronic NA treatment. Furthermore, no changes in subcellular fraction-associated ecNOS were found; this indirectly shows that chronic NA did not cause phosphorylation of the enzyme. Moreover, [3H]L-arginine transport through the plasma membrane was conserved in chronically NA-treated cells. The data demonstrate that prolonged in vitro exposure to pathologic CHF-like NA does not affect the L-arginine: NO pathway in human endothelial cells.
KW - Endothelial cells
KW - Nitric oxide
KW - Nitric oxide synthase
KW - Noradrenaline
UR - http://www.scopus.com/inward/record.url?scp=0031667479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031667479&partnerID=8YFLogxK
U2 - 10.1007/s003950050092
DO - 10.1007/s003950050092
M3 - Article
C2 - 9782366
AN - SCOPUS:0031667479
VL - 93
SP - 250
EP - 256
JO - Basic Research in Cardiology
JF - Basic Research in Cardiology
SN - 0300-8428
IS - 4
ER -