Effects of cigarette smoking on the human urinary proteome

Luisa Airoldi, Cinzia Magagnotti, Angela Rita Iannuzzi, Cristina Marelli, Renzo Bagnati, Roberta Pastorelli, Alessandro Colombi, Stefano Santaguida, Chiara Chiabrando, Silvia Schiarea, Roberto Fanelli

Research output: Contribution to journalArticle


In this pilot study we used a proteomic approach to compare the urinary protein patterns of healthy smokers and non-smokers. Proteins were resolved by two-dimensional gel electrophoresis and identified by mass spectrometry. The relative abundance of three inflammatory proteins (S100A8, inter-alpha-trypsin inhibitor heavy chain 4, CD59) and that of two isoforms of pancreatic alpha amylase was significantly higher in smokers. Zinc-alpha-2-glycoprotein was the only protein down-regulated in smokers. Its abundance was significantly correlated with urinary glucocorticoids. Most of the proteins identified may be non-specific biomarkers of tobacco effects, since they are involved in inflammatory responses associated with several diseases. Of greater interest are the changes in abundance of pancreatic alpha amylase and zinc-alpha-2-glycoprotein, which after proper validation, might be candidate biomarkers of diseases resulting from exposure to tobacco smoke. The data also show for the first time that smoking can affect the expression profile of urinary proteins.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Apr 10 2009


  • 2-Dimensional electrophoresis
  • Cigarette smoking
  • Mass spectrometry
  • Pathway analysis
  • Proteomics
  • Urine
  • Western blotting

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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  • Cite this

    Airoldi, L., Magagnotti, C., Iannuzzi, A. R., Marelli, C., Bagnati, R., Pastorelli, R., Colombi, A., Santaguida, S., Chiabrando, C., Schiarea, S., & Fanelli, R. (2009). Effects of cigarette smoking on the human urinary proteome. Biochemical and Biophysical Research Communications, 381(3), 397-402. https://doi.org/10.1016/j.bbrc.2009.02.055